Modulation of differentiation in vitro. I. Influence of the attachment surface on myogenesis

In Vitro
J P WahrmannA Macieira-Coelho

Abstract

Myogenic cells of the L6 line proliferate and fuse in culture to form myotubes that actively synthesize muscle-specific proteins such as myosin. We show that the expression of the differentiated phenotype can be influenced by the electrical charges of the substratum on which the cells were grown. Negatively charged surfaces did not influence the developmental program of the cells although positively charged ones interfered with myogenesis. The interaction operates primarily by interfering with the mitotic cycle, which is slowed down, with fusion which is blocked, and with myosin synthesis, which is reduced. Our results show that growth of the cells on positively charged surfaces prevents the switching of a large fraction of the population from a proliferative state to a differentiating program. We postulate that this interference might operate through the slowdown in DNA replication. The cell culture method described represents a good model for studying the different steps involved in the differentiation of L6 cells.

References

Dec 1, 1979·In Vitro·J R Florini, S B Roberts
May 1, 1979·Cell Biology International Reports·C DiatloffA Macieira-Coelho
May 1, 1976·Experimental Cell Research·J N KetleyG R Martin
May 1, 1979·Developmental Biology·P G Pauw, J D David
Mar 1, 1978·Developmental Biology·I R KonigsbergL O Mixter
Jun 1, 1978·Nature·J Folkman, A Moscona
Apr 25, 1975·Journal of Molecular Biology·C P Emerson, S K Beckner
Dec 1, 1977·The Journal of Cell Biology·N KalderonN B Gilula
Sep 1, 1971·Developmental Biology·I R Konigsberg
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W F LoomisD Luzzati
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A Macieira-Coelho, S Avrameas
Oct 1, 1972·Developmental Biology·B Paterson, R C Strohman
Feb 1, 1974·Developmental Biology·J L Doering, D A Fischman
Jun 1, 1974·Journal of Cellular Physiology·A Macieira-CoelhoS Avrameas
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·S D Hauschka, I R Konigsberg
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe
Apr 1, 1969·Developmental Biology·M Nameroff, H Holtzer

❮ Previous
Next ❯

Related Concepts

Related Feeds