PMID: 2510826Nov 20, 1989Paper

Modulation of DNA synthesis in cultured muscle cells by 1,25-dihydroxyvitamin D-3

Biochimica Et Biophysica Acta
L DrittantiR Boland

Abstract

Biphasic effects of 1,25-dihydroxyvitamin D-3 on DNA synthesis were shown in primary cultured (24 h) chick embryo myoblasts exposed to physiological concentrations of the hormone. The sterol stimulated [3H]thymidine incorporation into DNA in proliferating myoblasts, e.g., at early stages of culture prior to cell fusion or in high serum-treated cells. The opposite effects were observed during the subsequent stage of myoblast differentiation in low-serum media. The mitogenic effect of 1,25-dihydroxyvitamin D-3 was correlated with an increase in c-myc mRNA and a decrease in c-fos mRNA levels, whereas its inhibitory action on DNA synthesis was accompanied by increased myofibrillar and microsomal protein synthesis and an elevation of creatine kinase activity, the latter suggesting a stimulation of muscle cell differentiation by the sterol. These data are in agreement with the results of previous morphological studies. Treatment of myoblasts with the calcium ionophore X-537 A or the phorbol ester TPA caused only a transient stimulation of [3H]thymidine incorporation into DNA, which occurred earlier than the response elicited by 1,25-dihydroxyvitamin D-3, suggesting that changes in intracellular Ca2+ and kinase C activity are not majo...Continue Reading

References

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Jun 15, 1989·Biochimica Et Biophysica Acta·L N DrittantiA R de Boland
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Jan 1, 1972·The Journal of Cell Biology·M C O'Neill, F E Stockdale

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Citations

Sep 13, 2012·Calcified Tissue International·Lisa Ceglia, Susan S Harris
Sep 27, 2013·Clinical Drug Investigation·Atsushi MoriShigeru Kohno
Mar 1, 1996·Domestic Animal Endocrinology·M V DodsonS G Velleman
Jan 19, 2013·The Journal of Endocrinology·Kevin J P RyanJohn M Brameld
Dec 28, 2010·Atherosclerosis·Ankur Gupta, Paul D Thompson
Apr 30, 2010·Current Rheumatology Reports·Nancy E Lane
Nov 5, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Ana P IrazoquiClaudia G Buitrago
Sep 10, 2014·BioMed Research International·Akira Wagatsuma, Kunihiro Sakuma
Aug 30, 2008·Molecular Aspects of Medicine·Lisa Ceglia
Jan 9, 2020·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Tomomi OkuboKatsuhiko Iwakiri
Mar 29, 2016·Journal of Cellular Physiology·K van der MeijdenR T Jaspers
Jul 29, 2020·Nutrients·Tohru HosoyamaKen Watanabe
Jun 1, 1992·The American Journal of Physiology·V L Akerstrom, M R Walters
Sep 1, 1994·Cellular Signalling·A R De Boland, R L Boland

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