Modulation of E2F activity via signaling through surface IgM and CD40 receptors in WEHI-231 B lymphoma cells.

The Journal of Biological Chemistry
E W LamG G Klaus

Abstract

Stimulation of the phenotypically immature B cell lymphoma WEHI-231 with anti-IgM induces G1 arrest followed by apoptotic cell death, which can be reversed by stimulation via the CD40 receptor. Here, we show that cells expressing bcl-xL (WEHI-bcl-xL) arrest at G0/G1 following culture with anti-IgM but do not undergo apoptosis. These arrested cells can be induced to reenter the cell cycle by ligation of CD40. We have therefore used these cells as a model to study the regulation of the transcription factor E2F, which is critically involved in transit through the cell cycle. We found that anti-IgM treatment induces the appearance of an inhibitory DNA binding complex containing the pRB-related pocket protein p130 together with E2F and a concomitant decrease in "free" E2F, consisting of E2F1 and its partner DP1; these effects were reversed following stimulation via CD40. These changes in free E2F levels were regulated by changes in E2F1 gene transcription, which is at least partly a result of control of E2F1 promoter activity through its E2F binding sites. Transient transfection experiments showed that either E2F1 or the viral oncoprotein E1A, which sequesters pocket proteins, including p130, overcame anti-IgM-induced cell cycle arr...Continue Reading

References

Dec 1, 1992·European Journal of Immunology·M A Valentine, K A Licciardi
Aug 1, 1990·European Journal of Immunology·J Hasbold, G G Klaus
Jun 1, 1990·European Journal of Immunology·L E BenhamouP Sarthou
Dec 21, 1989·Nature·Y J LiuI C MacLennan
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D Nemazee, K Buerki
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B L PikeG J Nossal
Jan 1, 1995·Advances in Immunology·C C GoodnowK P Shokat
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M ErisP D Hodgkin
Jan 1, 1994·Annual Review of Immunology·J BanchereauS Saeland
Jul 28, 1995·Gene·E W LamR J Watson
May 1, 1995·Molecular and Cellular Biology·C L WuJ A Lees
Jun 1, 1995·Molecular and Cellular Biology·E M HijmansR Bernards
May 1, 1995·European Journal of Immunology·F E LundM C Howard
Jun 1, 1995·Genes & Development·R L BeijersbergenR Bernards
Dec 1, 1994·Current Opinion in Cell Biology·E W Lam, N B La Thangue
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·C SardetR A Weinberg
Nov 15, 1994·Genes & Development·D GinsbergD M Livingston
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Q QinP D Adams
Mar 1, 1994·Molecular and Cellular Biology·Y LiP J Farnham
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Wu, A J Levine
Dec 1, 1993·Molecular and Cellular Biology·J A LeesK Helin
Jan 1, 1994·Molecular and Cellular Biology·B ShanW H Lee
Jan 1, 1994·The Journal of Experimental Medicine·D A Fulcher, A Basten
Nov 1, 1993·Molecular and Cellular Biology·M A Ikeda, J R Nevins

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Citations

Oct 14, 2000·Cell Research·D Donjerković, D W Scott
Jun 17, 2004·Science's STKE : Signal Transduction Knowledge Environment·Margaret M Harnett
Feb 15, 2005·Neurobiology of Aging·Natividad de las CuevasAngeles Martín-Requero
Apr 17, 1999·The Journal of Biological Chemistry·J van der SmanE W Lam
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nongliao ZhuMichael R Gold
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ravichandran PanchanathanDivaker Choubey
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stephen B GauldMargaret M Harnett

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