Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1 beta and lipopolysaccharide in C6 rat glioma cells

Neuropeptides
A KugayaS Yamawaki

Abstract

We have investigated the effects of interleukin (IL)-1 beta and lipopolysaccharide (LPS) on endothelin (ET)-induced intracellular Ca2+ rise in C6 rat glioma cells in order to study the mechanisms of their effects on Ca2+ signaling systems. Pretreatment with IL-1 beta (10(3) U/mL) and LPS (1 microgram/mL) for 24 h significantly inhibited 100 nM ET-1-induced increase in intracellular Ca2+ either in the presence or absence of external Ca2+. Their inhibitory effects were in dosedependent (IL-1 beta; 50-1000 U/mL, LPS; 10-1000 ng/mL) and time-dependent (12-24 h) manners. A tyrosine kinase antagonist genistein (50 microM) but not a protein kinase C inhibitor H7 (30 microM) prevented the inhibition of the ET response by IL-1 beta and LPS. These results suggest that activation of tyrosine kinase may be essential for the inhibition of the ET receptor-mediated Ca2+ signaling systems by IL-1 beta and LPS.

References

Aug 1, 1992·Journal of Cellular Physiology·J R BetheaE N Benveniste
Dec 14, 1990·Biochemical and Biophysical Research Communications·D W Coyne, A R Morrison
Jan 30, 1990·Biochemical and Biophysical Research Communications·A Ohnishi-SuzakiS Kimura
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M W MacCumberS H Snyder
Jun 1, 1990·Journal of Neurochemistry·R MarsaultC Frelin
Jan 1, 1990·Life Sciences·D L Palazzolo, S K Quadri
Jan 16, 1989·Biochemical and Biophysical Research Communications·P A MarsdenT A Brock
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A InoueT Masaki

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Citations

Jan 24, 2009·International Journal of Cancer. Journal International Du Cancer·Yun ChenSheng-Hong Tseng
Apr 11, 2000·Journal of Molecular and Cellular Cardiology·L PiacentiniJ S Karliner

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