Modulation of excitatory synaptic transmission by nociceptin in superficial dorsal horn neurones of the neonatal rat spinal cord

British Journal of Pharmacology
J T LiebelH U Zeilhofer

Abstract

1. The modulatory actions of nociceptin/orphanin FQ on excitatory synaptic transmission were studied in superficial dorsal horn neurones in transverse slices from 7 to 14 day old rats. 2. Glutamatergic excitatory postsynaptic currents (e.p.s.cs) were recorded from the somata of the neurones in the whole-cell patch-clamp configuration. E.p.s.cs were evoked by extracellular electrical stimulation (100 microns, 3-10 V) of the ipsilateral dorsal root entry zone by use of a glass electrode. E.p.s.cs with constant short latency (< 2.3 ms) and with no failures upon stimulation were assumed to be monosynaptic. These e.p.s.cs occurred with an average latency of 1.72 +/- 0.098 ms and exhibited a fast decay with a time constant, tau, of 4.8 +/- 0.53 ms (n = 30). 3. Nociceptin reversibly reduced the amplitudes of e.p.s.cs in a concentration-dependent manner in 25 out of 27 cells tested. Average maximum inhibition was 51.6 +/- 5.7% (mean +/- s.e.mean; n = 9), at concentrations > 3 microM. EC30 was 485 +/- 47 nM and the Hill coefficient was 1.29 +/- 0.09. 4. Inhibition of synaptic transmission by nociceptin (10 microM) was insensitive to the non-specific opioid receptor antagonist naloxone (10 microM) indicating that nociceptin did not act v...Continue Reading

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Jan 31, 2004·Brain Research·Maria Luisa SotgiuGabriele E M Biella
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