Modulation of expression and assembly of vinculin during in vitro fibrillar collagen-induced angiogenesis and its reversal

Experimental Cell Research
C F DeroanneC M Lapiere

Abstract

A model of collagen-induced in vitro angiogenesis was used to investigate the modulation of expression and assembly of focal adhesion plaque-associated proteins during the process of differentiation. Human umbilical vein endothelial cells (HUVEC), first attached on an adhesive substratum (gelatin-, fibronectin-, or laminin-coated dish) or adherent collagen gel and then covered by an overlaying collagen get, organized within 3-4 days in tube-like structures (TLS). Removing the overlaying collagen gel from fully differentiated HUVEC induced a reversion of the process and HUVEC returned to a monolayer pattern. Modulations of focal adhesion-associated proteins occurring in HUVEC during the in vitro differentiation process and its reversal were investigated by Western blot analysis. A significant decrease of expression of vinculin, the integrin alpha2 subunit, talin, alpha-actinin, and actin was observed in TLS whereas the amount of FVIII-related antigen did not vary as compared to control monolayer cultures. During reversal, all the reduced proteins were markedly reexpressed. Human skin fibroblasts (HSF), submitted to the same experimental conditions, did not form TLS. Most of the focal adhesion proteins in HSF were similarly modul...Continue Reading

Citations

Jul 23, 2002·Molecular Biology of the Cell·John O ConnollyAlan Hall
May 3, 2008·Genes & Development·Xiaoming ZhouStephen J Weiss
Jul 8, 2009·Angewandte Chemie·Takahiro MuraokaSamuel I Stupp
Jun 6, 2016·Drug Discovery Today·Clara Sanz-Nogués, Timothy O'Brien
Jun 25, 2010·Cellular and Molecular Life Sciences : CMLS·J DubailA Colige
Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A F BlackF A Auger
Mar 5, 2003·Journal of Cell Science·Christophe DeroanneJacques Pouysségur

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.