Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids

Scientific Reports
Marta MoskotMagdalena Gabig-Cimińska

Abstract

Flavonoids were found previously to modulate efficiency of synthesis of glycosaminoglycans (GAGs), compounds which are accumulated in cells of patients suffering from mucopolysaccharidoses (MPSs). The aim of this work was to determine effects of different flavonoids (genistein, kaempferol, daidzein) used alone or in combinations, on expression of genes coding for proteins involved in GAG metabolism. Analyses with DNA microarray, followed by real-time qRT-PCR revealed that genistein, kaempferol and combination of these two compounds induced dose- and time-dependent remarkable alterations in transcript profiles of GAG metabolism genes in cultures of wild-type human dermal fibroblasts (HDFa). Interestingly, effects of the mixture of genistein and kaempferol were stronger than those revealed by any of these compounds used alone. Similarly, the most effective reduction in levels of GAG production, in both HDFa and MPS II cells, was observed in the presence of genistein, keampferol and combination of these compounds. Forty five genes were chosen for further verification not only in HDFa, but also in MPS II fibroblasts by using real-time qRT-PCR. Despite effects on GAG metabolism-related genes, we found that genistein, kaempferol and ...Continue Reading

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Citations

Jul 26, 2015·Metabolic Brain Disease·Marta MoskotMagdalena Gabig-Cimińska
Aug 9, 2015·Biochemical and Biophysical Research Communications·Michał GrabowskiZyta Banecka-Majkutewicz
Dec 29, 2016·Medicinal Research Reviews·Giancarlo Ghiselli
Jan 19, 2018·International Journal of Molecular Sciences·Marta MoskotMagdalena Gabig-Cimińska
Feb 13, 2018·Neuromolecular Medicine·Karolina PierzynowskaGrzegorz Węgrzyn
Nov 18, 2017·Nature Reviews. Drug Discovery·Frances M Platt
Feb 20, 2020·International Journal of Molecular Sciences·Francesca D'AvanzoRosella Tomanin
Feb 23, 2020·Pharmaceutics·Anna KwiecieńMaria Walczak
Apr 16, 2020·Journal of Cellular and Molecular Medicine·Bingyu ShenGuowen Liu
Nov 18, 2018·Italian Journal of Pediatrics·Simona FecarottaGiancarlo Parenti
Jul 3, 2019·International Journal of Molecular Sciences·Fabiane Valentini Francisqueti-FerronCamila Renata Correa
Feb 15, 2018·PloS One·Elwira SmolińskaMagdalena Gabig-Cimińska
Jul 28, 2017·Metabolic Brain Disease·Zyta Banecka-MajkutewiczBogdan Banecki
Dec 2, 2020·Fundamental & Clinical Pharmacology·Anaelle da CostaMathieu Charvériat
Aug 3, 2019·European Journal of Medicinal Chemistry·Lucia BiasuttoMario Zoratti
May 1, 2021·International Journal of Molecular Sciences·Graciela ArgüelloSilvana Zanlungo
Jun 27, 2018·Molecular Therapy. Methods & Clinical Development·Valeria De PasqualeLuigi Michele Pavone
May 29, 2021·Journal of Inherited Metabolic Disease·Arunabha GhoshSimon A Jones
Jul 3, 2021·International Journal of Molecular Sciences·Valeria De PasqualeLuigi Michele Pavone
Nov 4, 2020·Current Pharmaceutical Design·Francyne KubaskiRoberto Giugliani

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Datasets Mentioned

BETA
GSE43692

Methods Mentioned

BETA
Assay
Reverse Transcription PCR

Software Mentioned

Illumina® BeadStudio Data Analysis
GOrilla
Gene Set Enrichment Analysis ( GSEA )

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