Feb 17, 2006

Modulation of FGF-2 binding to chondrocytes from the developing growth plate by perlecan

Matrix Biology : Journal of the International Society for Matrix Biology
Prasanthi GovindrajJohn R Hassell


FGF-2 is a regulator of chondrocyte proliferation in the developing growth plate and has been shown to bind to perlecan, a heparan sulfate proteoglycan. We evaluated the effect of perlecan isolated from the growth plate on the binding of FGF-2 to its low and high affinity receptors on resting and proliferating chondrocytes. Chondrocytes were isolated by pronase/collagenase digestion of 1 mm thick slices from the resting and proliferating zones of fetal bovine ribs and were plated in serum-free DMEM. Chondrocytes maintained their zone-specific level of DNA and matrix synthesis over a two-day culture period. The collagen, aggrecan, and perlecan components of the matrix produced were associated with the cell layer and were secreted into the medium. Most of the perlecan made by the chondrocytes was secreted into the medium. Western blots showed medium perlecan to contain two high molecular weight core proteins and overlay assays showed only the large core protein bound FGF-2. Cell layer perlecan contained only the smaller core protein. Immunoprecipitation assays of media showed that the medium perlecan bound (125)I-FGF-2, that the bound FGF-2 was eluted from perlecan by 2 M NaCl at pH 7.4, and that this binding was eliminated by pr...Continue Reading

Mentioned in this Paper

Bone Structure of Rib
Iodine Isotopes
Chondrocyte Proliferation
Cell Culture Techniques
ACAN gene
Bos taurus
Plasma Protein Binding Capacity
Cell Proliferation

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