Modulation of fibroblast proliferation and transformation by activated macrophages during postoperative peritoneal reepithelialization

American Journal of Obstetrics and Gynecology
H OritaG S Dizerega

Abstract

We studied the modulation of fibroblast proliferation and transformation by postoperative macrophages. One group of rabbits underwent resection and reanastomosis of the small bowel, after which macrophages were collected by peritoneal lavage. A second group of rabbits underwent peritoneal wall abrasion followed by collection of local fibroblast on postoperative days 4 and 8. Postoperative macrophages were added to five culture dishes containing fibroblasts. After 24 hours, tritiated thymidine was added to the culture dishes and incubated overnight. In two other dishes, which were incubated for up to 8 days, a 24-hour pulse of tritiated thymidine was added before culture termination. Postoperative day 4 fibroblasts demonstrated a greater increase in cell number during the culture interval compared to fibroblasts collected on postoperative day 8. By the second day of coculture with macrophages collected from different postoperative days, tritiated thymidine incorporation by day 4 fibroblasts was suppressed, especially by postoperative day 7 macrophages. Thereafter, a stimulation in tritiated thymidine uptake was found. In contrast, tritiated thymidine uptake by day 8 fibroblasts was accelerated by coculture with macrophages, espe...Continue Reading

References

Apr 1, 1973·The British Journal of Surgery·A T Raftery

❮ Previous
Next ❯

Citations

Dec 22, 1999·The American Journal of Emergency Medicine·D C Lee, J H Valente
Nov 1, 1988·The Journal of Surgical Research·M FukasawaG S diZerega
Jul 1, 1989·The Journal of Surgical Research·M FukasawaG S DiZerega
Nov 1, 1992·The Journal of Surgical Research·K E Rodgers, G S diZerega
Sep 1, 1992·The Journal of Surgical Research·S KuraokaG S diZerega
Oct 1, 1988·The Journal of Surgical Research·M FukasawaG S diZerega
Feb 1, 1995·Journal of Assisted Reproduction and Genetics·S D PrienR H Messer
Dec 1, 1987·The Journal of Surgical Research·M FukasawaG S diZerega
Mar 1, 1989·The Journal of Surgical Research·M FukasawaG S DiZerega

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.