Modulation of glial and neuronal migration by lipocalin-2 in zebrafish.

Immune Network
Ho KimKyoungho Suk

Abstract

Glial cells are involved in immune and inflammatory responses in the central nervous system (CNS). Glial cells such as microglia and astrocytes also provide structural and functional support for neurons. Migration and morphological changes of CNS cells are associated with their physiological as well as pathological functions. The secreted protein lipocalin-2 (LCN2) has been previously implicated in regulation of diverse cellular processes of glia and neurons, including cell migration and morphology. Here, we employed a zebrafish model to analyze the role of LCN2 in CNS cell migration and morphology in vivo. In the first part of this study, we examined the indirect effect of LCN2 on cell migration and morphology of microglia, astrocytes, and neurons cultured in vitro. Conditioned media collected from LCN2-treated astrocytes augmented migration of glia and neurons in the Boyden chamber assay. The conditioned media also increased the number of neuronal processes. Next, in order to further understand the role of LCN2 in the CNS in vivo, LCN2 was ectopically expressed in the zebrafish spinal cord. Expression of exogenous LCN2 modulated neuronal cell migration in the spinal cord of zebrafish embryos, supporting the role of LCN2 as a ...Continue Reading

References

Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Feb 20, 1998·Neuroscience Letters·C H KimT Hirano
Feb 24, 2001·Annals of the New York Academy of Sciences·W ArakiT Tabira
Nov 28, 2002·Molecular Cell·Jun YangJonathan Barasch
Feb 16, 2005·The Journal of Clinical Investigation·Kiyoshi MoriJonathan Barasch
Aug 3, 2005·The Biochemical Journal·Zhimin TongJames P Kehrer
Dec 27, 2005·Cell·Laxminarayana R DevireddyMichael R Green
Jun 8, 2007·Breast Cancer Research and Treatment·Maret BauerAndreas Friedl
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shinrye LeeKyoungho Suk
Oct 17, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kristen M KwanChi-Bin Chien
Oct 30, 2007·Nature Neuroscience·Uwe-Karsten Hanisch, Helmut Kettenmann
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Gwenn A Garden, Thomas Möller
Mar 5, 2008·The Journal of Clinical Investigation·Amanda M NelsonDiane M Thiboutot
Mar 28, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Szu-Yi ChouYijuang Chern
Aug 30, 2008·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Davide BolignanoMichele Buemi
Jan 9, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shinrye LeeKyoungho Suk
Feb 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jiang YangMarsha A Moses
Mar 24, 2009·Annual Review of Immunology·Richard M Ransohoff, V Hugh Perry

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Citations

Jun 10, 2014·Nature Communications·Qi MiaoHoang Nguyen
Jan 20, 2016·Inflammopharmacology·Yuan ZhangHonghong Yao
Dec 28, 2012·Biochimica Et Biophysica Acta·Mithilesh Kumar JhaKyoungho Suk
Jan 16, 2016·Neurology. Neuroimmunology and Neuroinflammation·Faiez Al NimerFredrik Piehl
Oct 15, 2013·Proteomics·Mithilesh Kumar JhaKyoungho Suk
Jan 21, 2014·Experimental Neurology·Mithilesh Kumar JhaKyoungho Suk
Dec 17, 2014·Neuroscience and Biobehavioral Reviews·Mithilesh Kumar JhaKyoungho Suk
Jul 27, 2012·Therapeutic Delivery·Bruno Sarmento, José das Neves
Feb 19, 2013·Expert Review of Proteomics·Mithilesh Kumar Jha, Kyoungho Suk

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

SPSS

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