Modulation of glucose uptake in a human choriocarcinoma cell line (BeWo) by dietary bioactive compounds and drugs of abuse

Journal of Biochemistry
João R AraújoFátima Martel

Abstract

The aim of this work was to investigate the putative modulation of glucose uptake in trophoblast cells by several dietary compounds and by drugs of abuse. For this, the acute (26 min) and chronic (48 h) effect of these substances on the apical uptake of 3H-2-deoxy-D-glucose (3H-DG) by a human choriocarcinoma cell line (BeWo) was determined. 3H-DG apical uptake by BeWo cells was time dependent, displayed saturable kinetics (Vmax=1210+/-29 nmol mg protein(-1) 6 min(-1) and Km=13.4+/-0.5 mM) and was insulin-insensitive and cytochalasin B-sensitive (by up to 60%). Acutely, acetaldehyde (30-100 mM), resveratrol, xanthohumol, epigallocatechin-3-gallate (100 microM), chrysin and quercetin (10-100 microM) decreased 3H-DG apical uptake, whereas rutin, catechin (10-100 microM), epicatechin (100 microM) and ethanol (10 mM) increased it. Quercetin and xanthohumol seem to be non-competitive inhibitors of 3H-DG apical uptake, whereas both epigallocatechin-3-gallate and acetaldehyde decreased both the Km and Vmax values. Chronically, rutin and myricetin increased the apical uptake of 3H-DG both isolated (0.1-1 microM) and in combination (both at 1 microM), whereas theophylline (0.1-1 microM) and amphetamine, 3,4-methylenedioxymethamphetamine ...Continue Reading

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Citations

Nov 22, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Pedro GonçalvesFátima Martel
Feb 3, 2011·The Journal of Membrane Biology·Pedro GonçalvesFátima Martel
Jun 29, 2013·Reproductive Toxicology·João Ricardo AraújoFátima Martel
Apr 16, 2010·Nutrition Research Reviews·Fátima MartelConceição Calhau
Aug 27, 2013·The Journal of Membrane Biology·Manuela MeirelesAna Faria
Sep 29, 2009·Molecular Nutrition & Food Research·Ana FariaConceição Calhau
Jul 11, 2013·The Journal of Pharmacy and Pharmacology·Virginia Demarchi KappelFátima Regina Mena Barreto Silva
Mar 23, 2011·Nutrition Research·João R AraújoFátima Martel
Nov 8, 2014·Human Reproduction Update·Christina LyAndrée Gruslin
Jan 13, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ming LiuJinlai Miao
Mar 17, 2011·American Journal of Physiology. Endocrinology and Metabolism·Huika LiRobin H Mortimer
Jan 13, 2021·Cancers·Aranka BrockmuellerMehdi Shakibaei
Apr 17, 2021·Cancer Cell International·Marjan TalebiSaeed Samarghandian

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