Modulation of glutamate release from rat hippocampal synaptosomes by nitric oxide

Nitric Oxide : Biology and Chemistry
S M SequeiraC M Carvalho

Abstract

We used hippocampal synaptosomes to study the effect of NO originating from NO donors and from the activation of the NO synthase on the Ca2+-dependent release of glutamate due to 4-aminopyridine (4-AP) depolarization. We distinguished between the effects of NO on the exocytotic and on the carrier-mediated release of glutamate, which we found to be related to an increase in cGMP content and to a reduction of the ATP/ADP ratio, respectively. The NO donor hydroxylamine, at concentrations < or = 0.3 mM, inhibited the Ca2+-dependent glutamate release evoked by 4-AP, and addition of the NO donor, NOC-7, had a similar effect, which was reversed by the NO scavenger, carboxy-PTIO. Increasing the activity of NO synthase by addition of L-arginine also led to a decrease in the Ca2+-dependent release of glutamate induced by 4-AP, and this effect was reversed by inhibiting NO synthase with NG-nitro-L-arginine. This depression of the exocytotic release of glutamate was accompanied by an increase in cGMP levels due to the stimulation of soluble guanylyl cyclase by NO, produced either by the NO donors (hydroxylamine <0.3 mM) or by the endogenous NO synthase, but no significant decrease in ATP/ADP ratio was observed. However, at concentrations >...Continue Reading

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Citations

Jul 19, 2002·Journal of Neurochemistry·A M Michela Di StasiMaurizio Minetti
Jun 16, 2010·Nitric Oxide : Biology and Chemistry·Guy C Brown
Nov 28, 2006·Nitric Oxide : Biology and Chemistry·Hiroyasu TsutsukiHideshi Ihara
Dec 18, 2004·Journal of Neuroscience Research·Ravinder PannuInderjit Singh
Aug 24, 2005·Progress in Neurobiology·F X GuixF J Muñoz
Sep 17, 2009·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Burcak BilginerNejat Akalan
Oct 6, 2012·The Journal of Toxicological Sciences·Pınar Yamantürk-ÇelikLütfiye Eroğlu
Jun 14, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kirsten S EvonukTara M DeSilva
Feb 22, 2012·Antioxidants & Redox Signaling·Alexander A MonginDavid Jourd'heuil
Dec 3, 2014·The Journal of Experimental Biology·Natalie L Pitts, Donald L Mykles

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