PMID: 8602341Dec 1, 1995Paper

Modulation of glycosidase and protease activities by chemostat growth conditions in an endocarditis strain of Streptococcus sanguis

Oral Microbiology and Immunology
J A MayoK W Knox

Abstract

The effects of growth conditions on the properties of the endocarditis-producing oral bacterium Streptococcus sanguis FSS2 were studied. This strain produces a variety of proteases and glycosidases, including a thrombin-like activity that is a potential virulence factor for endocarditis. Cultures were grown with limiting glucose or galactose in chemostats over a range of dilution rates and pH levels, and the following activities were measured at pH 7.5: thrombin-like, Hageman factor-like, N-acetyl-beta-D-galactosaminidase, beta-D-glucosidase, and beta-D-galactosidase. At growth pH 6.5, specific activities generally decreased as the dilution rate increased from 0.05 to 0.40 h(-1). At a dilution rate of 0.1 h(-1), specific activities generally were highest at growth pH 6.5 and lower and approximately equal at growth pH 5.5 and 7.5. The major exception was the thrombin-like activity, for which the specific activity at growth pH 7.5 was approximately 5-fold higher than at growth pH 5.5. Hageman factor-like activity was apparently glucose catabolite repressible, as its activity was 3-fold higher in galactose cultures. The measured activities changed as functions of growth conditions and thus were modulated by environment. Environmen...Continue Reading

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Citations

Jan 18, 2002·Infection and Immunity·J M GoldsteinJ Travis
Jan 25, 2005·Infection and Immunity·J M GoldsteinJ Travis
Jun 5, 2004·Biochemical and Biophysical Research Communications·Derek W S HartyNicholas A Jacques
May 20, 2016·Archives of Pharmacal Research·Gloria Gutiérrez-Venegas, Zeltzin González-Rosas
Jan 1, 1996·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M C Herzberg
Apr 1, 1997·Advances in Dental Research·J Carlsson
Apr 4, 2000·Biotechnology and Bioengineering·M G WiebeA P Trinci

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