Modulation of granulosa cell deoxyribonucleic acid synthesis and differentiation by activin

Endocrinology
F Miró, S G Hillier

Abstract

FSH stimulates follicular growth through inducing granulosa cell proliferation. Our working hypothesis is that mitogenesis is facilitated by a locally produced growth/differentiation factor(s) that modulates FSH action in developing granulosa cells. The present study was undertaken to examine the effect of the related gonadal peptides activin and inhibin on granulosa cell proliferation. Monolayer cultures of granulosa cells isolated from preantral/early antral follicles in immature rat ovaries were established by incubation overnight in serum-free medium 199. Treatment was initiated with serum-free medium containing recombinant human (rh) activin and/or rh-inhibin in the presence or absence of FSH. After incubation for 18 h, medium was collected for progesterone determination (as a marker of cell differentiation), and the cell monolayers were incubated for 2 h in the presence of [3H]thymidine to measure DNA synthesis. Activin dose dependently (1-100 ng/ml) stimulated DNA synthesis (minimal effective dose, 1 ng/ml), whereas inhibin or FSH alone was without effect. When activin (1 ng/ml), but not inhibin, was present with FSH, the gonadotropin caused dose-dependent increases in [3H]DNA synthesis over a wide range of FSH concentra...Continue Reading

Citations

May 6, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Craig N HarveyMehmet Uzumcu
Jun 18, 2004·Reproductive Biology and Endocrinology : RB&E·Anthony J Zeleznik
Apr 14, 2006·Reproductive Biology and Endocrinology : RB&E·Fiona H Thomas, Barbara C Vanderhyden
Jun 23, 2001·Molecular and Cellular Endocrinology·S G Hillier
Apr 10, 2013·Molecular and Cellular Endocrinology·Kirsten J McTavishShunichi Shimasaki
Sep 29, 2004·Molecular and Cellular Endocrinology·Shanthi MuttukrishnaIan Sargent
Dec 18, 2013·Biochemical and Biophysical Research Communications·Jung-Chien ChengPeter C K Leung
Nov 4, 2006·Molecular and Cellular Endocrinology·M Ram Sairam, P Suresh Babu
Aug 12, 2009·Developmental Biology·Michelle MyersStephanie A Pangas
May 30, 1998·Biochemical Pharmacology·T K Woodruff
Jun 22, 2016·Molecular and Cellular Endocrinology·Maria K HerndonMary Hunzicker-Dunn
Dec 18, 2001·Molecular Reproduction and Development·R van den Hurk, S A Van de Pavert
Jul 10, 2018·Endocrinology·Alfredo Ulloa-AguirrePascale Crépieux
Sep 24, 2004·Biology of Reproduction·Sheila A Sweeney, Patricia A Johnson
Nov 1, 2018·Cell and Tissue Research·Fumiya OjimaSumio Takahashi
Aug 26, 1998·Biology of Reproduction·R L Robker, J S Richards
Aug 22, 2001·Biology of Reproduction·A J DulebaR Z Spaczynski
Jan 26, 2006·Endocrine Reviews·Nick S MacklonBart C J M Fauser
Jan 19, 1999·Molecular Endocrinology·D A DeMannoM Hunzicker-Dunn
Aug 19, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C Peng, S T Mukai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.