Modulation of gurken translation by insulin and TOR signaling in Drosophila.

Journal of Cell Science
Scott B FergusonT Schüpbach

Abstract

Localized Gurken (Grk) translation specifies the anterior-posterior and dorsal-ventral axes of the developing Drosophila oocyte; spindle-class females lay ventralized eggs resulting from inefficient grk translation. This phenotype is thought to result from inhibition of the Vasa RNA helicase. In a screen for modifiers of the eggshell phenotype in spn-B flies, we identified a mutation in the lnk gene. We show that lnk mutations restore Grk expression but do not suppress the persistence of double-strand breaks nor other spn-B phenotypes. This suppression does not affect Egfr directly, but rather overcomes the translational block of grk messages seen in spindle mutants. Lnk was recently identified as a component of the insulin/insulin-like growth factor signaling (IIS) and TOR pathway. Interestingly, direct inhibition of TOR with rapamycin in spn-B or vas mutant mothers can also suppress the ventralized eggshell phenotype. When dietary protein is inadequate, reduced IIS-TOR activity inhibits cap-dependent translation by promoting the activity of the translation inhibitor eIF4E-binding protein (4EBP). We hypothesize that reduced TOR activity promotes grk translation independent of the canonical Vasa- and cap-dependent mechanism. Th...Continue Reading

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Citations

Jul 19, 2013·Molecular Biology of the Cell·Marcio FonteneleHelena Araujo
Jun 3, 2016·Nature Reviews. Molecular Cell Biology·Cyril F BourgeoisDidier Auboeuf
Feb 7, 2017·Nature Genetics·Yogesh GoyalStanislav Y Shvartsman
Apr 18, 2017·Mechanisms of Ageing and Development·Yulia Gonskikh, Norbert Polacek
Dec 27, 2019·Molecular Biology of the Cell·Alexander O BradleyMargot E Quinlan
Jan 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Granton A JindalStanislav Y Shvartsman
Jan 6, 2015·Cytoskeleton·Batbileg BorMargot E Quinlan

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