Modulation of hematopoietic and endothelial cell differentiation from mouse embryonic stem cells by different culture conditions.

Blood
Wen Jie ZhangKyunghee Choi

Abstract

Embryonic stem (ES) cells can differentiate into many different somatic cells in culture. To better correlate hematopoietic and endothelial cell differentiation of ES cells in currently available protocols, we compared fetal liver kinase-1 (Flk-1)-, stem cell leukemia (Scl)-, and vascular endothelial-cadherin (VE-cadherin)-expressing cells generated in embryoid bodies (EBs) and on OP9 cells. We report that the kinetics of Scl and Flk-1 expression were similar in EBs and OP9 cells, although Flk-1 expression was extended on OP9 cells. CD45+ and Ter-119+ cells developed more efficiently in EBs, whereas VE-cadherin+ cells developed largely on OP9 cells. Cell sorting and replating studies showed that Scl+ cells, not Flk-1+ or VE-cadherin+ cells, were enriched for primitive and definitive hematopoietic progenitors. Our studies indicate that optimal hematopoietic and endothelial cell differentiation occur in EBs and on OP9 cells, respectively. Regardless of the culture systems used, Scl is the most relevant marker for enriching primitive and definitive hematopoietic progenitors.

References

Jan 1, 1993·Molecular and Cellular Biology·G KellerM V Wiles
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·D VittetP Huber
Dec 12, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T FujimotoS Nishikawa
Feb 16, 2002·Journal of Hematotherapy & Stem Cell Research·Kyunghee Choi
Oct 31, 2002·Development·Yun Shin ChungKyunghee Choi

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Citations

Feb 20, 2010·International Journal of Hematology·Hiroshi SakamotoMinetaro Ogawa
Jul 30, 2008·The Journal of Experimental Medicine·Hidekazu NishikiiHiromitsu Nakauchi
May 25, 2005·Stem Cells and Development·Nicanor I Moldovan
Oct 29, 2005·Blood·Abby L OlsenMitchell J Weiss
Dec 25, 2009·BMC Developmental Biology·Vesna Nikolova-KrstevskiPeter Oettgen
Nov 15, 2011·Biochemical and Biophysical Research Communications·Min Young LeeYibing Qyang
Jun 20, 2007·Experimental Hematology·Gregor A Prindull, Eitan Fibach
Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·X Li, L Claesson-Welsh
May 12, 2006·Periodontology 2000·Susan X Hsiong, David J Mooney
Aug 7, 2008·Differentiation; Research in Biological Diversity·Torsten SeifertStefan O Peters
Jan 21, 2009·Biomaterials·Devang K ThakorYasuhiko Tabata
Oct 16, 2009·Trends in Molecular Medicine·Derya TilkiSüleyman Ergün
Sep 5, 2008·Current Protocols in Stem Cell Biology·Yunglin D MaKyunghee Choi
Jan 30, 2008·Current Protocols in Cell Biology·Maxim A Vodyanik, Igor I Slukvin
Jan 1, 2010·Therapeutic Advances in Cardiovascular Disease·Catharina NesselmannGustav Steinhoff
Oct 16, 2013·The Journal of Cell Biology·Christian ElabdMichael J Conboy
Aug 26, 2015·Molecular and Cellular Biology·Felix LohmannJames J Bieker

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