Modulation of hippocampal norepinephrine release by cholinergic agonists is altered by AF64A lesion

Brain Research Bulletin
P E PotterC Gaughan

Abstract

The effect of lesioning hippocampal cholinergic neurons with the neurotoxin AF64A on the ability of cholinergic agonists to modulate stimulation-induced release of 3H-norepinephrine (NE) from rat hippocampal slices was studied. Rats received intracerebroventricular injections of either AF64A (ethylcholine mustard aziridinium, 2 nmol) or vehicle (sham operated). Six weeks after treatment, release of 3H-NE evoked by electrical stimulation (2 Hz, 2 min) in the presence or absence of cholinergic agonists and/or antagonists was measured. Activation of M2 receptors with oxotremorine (in the presence of the M1 antagonist pirenzepine) caused a small inhibition of NE release, which was abolished in hippocampi from AF64A-treated rats. The Kd for high-affinity binding of the selective M2 ligand [3H] AF-DX 384 was increased 10-fold in lesioned tissues. The M1 selective agonist McN-A-343 produced a significant enhancement of NE release, which was unchanged by AF64A lesion. Binding studies with [3H] pirenzepine showed no change in the affinity or number of M1 receptors. Nicotine also caused a significant enhancement of evoked NE release, but this effect was markedly reduced in tissues from AF64A-treated rats. AF64A treatment caused a twofold...Continue Reading

References

May 1, 1990·Trends in Pharmacological Sciences·C J FowlerR F Cowburn
Oct 26, 1988·European Journal of Pharmacology·R W Ransom, N L Deschenes
Nov 1, 1986·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·E K PerryA Fairbairn
Aug 1, 1984·Pharmacology, Biochemistry, and Behavior·L E JarrardA Levy
Jul 30, 1982·Science·R T BartusA S Lippa
Jan 1, 1995·Brain Research Bulletin·B Thorne, P E Potter
May 10, 1995·Annals of the New York Academy of Sciences·E S ViziA Lajtha

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Citations

Jul 14, 1999·Neurochemistry International·M McLaughlinJ McCulloch
Mar 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Li-Wei LinChi-Rei Wu
Jun 11, 2020·Advances in Wound Care·Olivia A CharltonAlan Cooper

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