Modulation of Hsf1 activity by novobiocin and geldanamycin

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Renaud CondeNick Ovsenek

Abstract

Since Hsp90 is a known modulator of HSF1 activity, we examined the effects of two pharmacological inhibitors of Hsp90, novobiocin and geldanamycin, on HSF1 DNA-binding activity in the Xenopus oocyte model system. Novobiocin exhibits antiproliferative activity in culture cells and interacts with a C-terminal ATP-binding pocket on Hsp90, inhibiting Hsp90 autophosphorylation. Treatment of oocytes with novobiocin followed by heat shock results in a dose-dependent decrease in HSF1 DNA-binding and transcriptional activity. Immunoprecipitation experiments demonstrate novobiocin does not alter HSF1 activity through dissociation of Hsp90 from either monomeric or trimerized HSF1, suggesting that the effect of novobiocin on HSF1 is mediated through alterations in Hsp90 autophosphorylation. Geldanamycin binds the N-terminal ATPase site of Hsp90 and inhibits chaperone activity. Geldanamycin treatment of oocytes resulted in a dose-dependent increase in stability of active HSF1 trimers during submaximal heat shock and a delay in disassembly of trimers during recovery. The results suggest that Hsp90 chaperone activity is required for disassembly of HSF1 trimers. The data obtained with novobiocin suggests the C-terminal ATP-binding activity of ...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·G L Firestone, S D Winguth
Nov 30, 1994·Experientia·A R HeydariA Richardson
Oct 15, 1996·The Biochemical Journal·R AlfieriA F Borghetti
May 30, 1997·The Journal of Biological Chemistry·P A MercierJ T Westwood
Aug 26, 1998·Molecular and Cellular Biology·A AliN Ovsenek
Sep 28, 2001·The Journal of Biological Chemistry·K F WinklhoferJ Tatzelt
Jan 10, 2002·Critical Care Medicine·Elisabeth S ChristiansIvor J Benjamin
Oct 14, 2003·Toxicology and Applied Pharmacology·Delphine WirthPascal Gustin
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marcin P MyckoKrzysztof W Selmaj
Dec 2, 2004·Biochimica Et Biophysica Acta·Claudia EsserJörg Höhfeld
Mar 15, 2005·Critical Reviews in Biochemistry and Molecular Biology·Daniel L RiggsDavid F Smith
May 18, 2005·Journal of the Neurological Sciences·Vittorio CalabreseD Allan Butterfield
Jun 22, 2005·The Journal of Biological Chemistry·Renaud CondeNick Ovsenek
Nov 22, 2005·The Journal of Surgical Research·Andrea A TandaraThomas A Mustoe
Aug 5, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Janine Voyer, John J Heikkila
Nov 11, 2008·Current Medicinal Chemistry·Alison Donnelly, Brian S J Blagg

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Citations

Apr 29, 2010·Current Oncology Reports·Jeffrey M HolzbeierleinGeorge Vielhauer
May 31, 2012·Journal of the American Chemical Society·Adam S DuerfeldtBrian S J Blagg
Apr 14, 2011·Journal of Natural Products·Jason DavenportRobert L Matts
Jul 24, 2010·Nature Reviews. Cancer·Jane TrepelLen Neckers
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Jacob VergheseKevin A Morano
Jan 5, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Len Neckers, Paul Workman
Jul 30, 2014·Expert Opinion on Therapeutic Targets·Laura J BlairChad A Dickey
Mar 4, 2014·Pharmacology & Therapeutics·Pierre-Simon BellayePhilippe Bonniaud
Nov 23, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Aditya AmbadePranoti Mandrekar
Dec 1, 2010·European Journal of Haematology·Alessandro AllegraCaterina Musolino
Jul 19, 2015·Endocrine-related Cancer·Margaret M CenteneraLisa M Butler
Jun 7, 2016·Cell Reports·Kaitlyn M GayvertOlivier Elemento
Jun 14, 2016·Biochimie·Sharad VermaAbhinav Grover
Jun 6, 2014·ACS Medicinal Chemistry Letters·Huiping ZhaoBrian S J Blagg
Apr 3, 2016·Chemistry : a European Journal·Katherine M ByrdBrian S J Blagg
Jan 1, 2011·Cancers·Aurelie De ThonelCarmen Garrido
Feb 7, 2015·Chemical Communications : Chem Comm·Maria StrocchiaGiuseppe Bifulco
Dec 6, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stuart K Calderwood
Apr 2, 2019·Expert Opinion on Therapeutic Targets·Toshiki KijimaYasuhisa Fujii
Oct 7, 2010·Expert Review of Respiratory Medicine·M Roshni RayBiao He
Mar 30, 2020·The FEBS Journal·Marie GuilbertEmmanuel Courtade
Jun 6, 2013·Future Medicinal Chemistry·Annerleim Walton-DiazMehdi Mollapour
Apr 26, 2013·Oncotarget·Yaoyu ChenWenlai Zhou
Sep 9, 2017·Nature Communications·Moore Z ChenDanny M Hatters
May 10, 2017·Frontiers in Neuroscience·Stuart K Calderwood, Ayesha Murshid
Jan 20, 2018·Nature Communications·Rebecca J WoodDanny M Hatters
May 16, 2018·Bioorganic & Medicinal Chemistry Letters·Zheng ZhangBrian S J Blagg
Aug 19, 2021·Naunyn-Schmiedeberg's Archives of Pharmacology·Lihong LiYajun Liu
Jul 27, 2018·Journal of Medicinal Chemistry·Mariarosaria FerraroGiorgio Colombo

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