Modulation of human airway smooth muscle biology by human adipocytes

Respiratory Research
Amanda GieslerParameswaran Nair

Abstract

Asthma and obesity, two growing epidemics worldwide, may share an underlying causal relationship. Airway hyperresponsiveness (AHR), a defining component of asthma, has been documented in both 'obese' animal models and non-asthmatic obese individuals. However, there is a paucity of evidence that obesity-derived factors directly affect human airway smooth muscles (ASM). Experiments were designed with primary ASM and adipocytes isolated from the same human tissue explants (n = 6). The modulatory effects of human adipocytes extracted from subcutaneous (extrathoracic) and visceral (intrathoracic) depots, on ASM biology was examined with respect to proliferation, migration, contractility and pro-inflammatory cytokine synthesis. Adipocyte-conditioned media as well as myocyte-adipocyte co-cultures failed to show any significant changes in the proliferative or migrational properties of the ASM. Adipocyte-conditioned media also had no effect on the contractility or relaxation of bovine tracheal muscle strips. In contrast, there was a moderate yet significant increase of IL-6 and eotaxin release by ASM incubated with adipocyte-conditioned media (P = 0.0035 and P = 0.0067, vs. control, respectively), thereby further consolidating the alter...Continue Reading

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Citations

Jul 21, 2020·Canadian Journal of Physiology and Pharmacology·Aamir MagzoubMohammed Shalayel
Feb 10, 2021·Respirology : Official Journal of the Asian Pacific Society of Respirology·Sarah Svenningsen, Parameswaran Nair
May 3, 2019·Respiratory Medicine·Mario CazzolaMaria Gabriella Matera
Jul 6, 2021·Mayo Clinic Proceedings·Sergio E ChiarellaY S Prakash

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Methods Mentioned

BETA
Assay

Software Mentioned

GraphPad Prism
Bio
Plex Manager

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