Modulation of human epidermal cell response to 2,3,7,8-tetrachlorodibenzo-p-dioxin by epidermal growth factor

Carcinogenesis
M A ReaR H Rice

Abstract

Cultured human epidermal cells were treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the presence or absence of epidermal growth factor (EGF). In both normal keratinocytes and a spontaneously immortalized keratinocyte (SIK) line, TCDD treatment in the absence of EGF induced a marked reduction in colony size and cell number, and it perturbed colony morphology. These effects were largely prevented by EGF, indicating that growth factor action in the cellular microenvironment may considerably modify TCDD action in target cells. Both TCDD and EGF substantially reduced expression of the differentiation markers keratin 1 and keratin 10 in the normal and immortalized cells, and did so in an additive fashion. The cells did not display a general loss of differentiated function, since several other markers, including involucrin, were little affected. EGF dramatically stimulated telomerase activity in SIK cultures, and TCDD prevented this action but not by reducing cell growth. However, EGF did not stimulate telomerase activity in normal human epidermal cells despite an evident increase in their growth. The growth factor stimulation of telomerase in the minimally deviated SIK line suggests that derepression of enzyme activity in ...Continue Reading

Citations

Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·M A Rea, R H Rice
Oct 11, 2003·Toxicology and Applied Pharmacology·Soma S Ray, Hollie I Swanson
Feb 16, 2000·Journal of Dermatological Science·M MatsuiA Aioi
Jun 17, 2006·The Journal of Investigative Dermatology·Miguel A ReaRobert H Rice
Jan 19, 2005·The Journal of Investigative Dermatology·Alexandra GeusauErwin Tschachler
Sep 16, 2006·Molecular Nutrition & Food Research·Stefanie Knerr, Dieter Schrenk
Apr 10, 2013·Toxicology and Applied Pharmacology·Marjorie A PhillipsRobert H Rice
Jul 16, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Filomena FioritoGiuseppe Iovane

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