Modulation of human fibroblast gap junction intercellular communication by hyaluronan

Journal of Cellular Physiology
K E Moyer, H P Ehrlich

Abstract

The composition of the extracellular matrix changes during dermal repair. Initially, hyaluronan (HA) concentration is high, however, by day 3, HA is eliminated. HA optimizes collagen organization within granulation tissue. One possible mechanism of HA modulation of collagen packing is through the promotion of gap junction intercellular communication (GJIC). Gap junctions are gated channels that allow rapid intercellular communication and synchronization of coupled cell activities. The gap junction channel is composed of connexin (Cx) proteins that form a gated channel between coupled cells. HA is reported to enhance Cx43 expression in transformed fibroblasts. GJIC was quantified by the scrape loading technique and reported as a coupling index. The coupling index for human dermal fibroblasts was 4.6 +/- 0.2, while the coupling index for fibroblasts treated with HA more than doubled to 10.6 +/- 0.7. By Western blot analysis no differences were appreciated in the protein levels of Cx43 or beta-catenin, a protein involved in the translocation of Cx to the cell surface. By immuno-histology Cx43 and beta-catenin were evenly distributed throughout the cell in controls, but in cells treated with HA these proteins were co-localized to t...Continue Reading

References

Sep 1, 1979·The Journal of Surgical Research·S A Alexander, R B Donoff
Sep 1, 1978·The Journal of Surgical Research·C N Bertolami, R B Donoff
Mar 1, 1992·Kidney International·G Gabbiani
Jun 26, 1992·Cell·R Nusse, H E Varmus
Dec 1, 1991·Experimental Eye Research·T NishidaT Otori
Oct 1, 1994·Cell Biology International·E BernardL Robert
Feb 9, 1996·Cell·N M Kumar, N B Gilula
Mar 1, 1996·The Journal of Surgical Research·E S KleinG Y Ben-Ari
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·B A RowningC A Larabell
May 1, 1997·Developmental Biology·C M HedgepethP S Klein
Jul 1, 1997·Journal of Internal Medicine·J R FraserU B Laurent
Apr 8, 1998·Current Opinion in Genetics & Development·K Willert, R Nusse
May 9, 1998·Journal of Pediatric Surgery·J A IoconoT M Krummel
Nov 10, 1998·Journal of Cellular Physiology·R M GrecoH P Ehrlich
Dec 9, 1998·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·J A IoconoH Paul
Apr 10, 1999·Molecular Biology of the Cell·F YokoyaY Yoneda
May 7, 1999·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·W Y Chen, G Abatangelo
Jan 22, 2000·The Journal of Clinical Investigation·Z AiG I Fishman
May 29, 2000·Journal of Cellular Physiology·H P EhrlichP Meda
Jul 18, 2000·American Journal of Physiology. Cell Physiology·K KoC McCulloch
Jan 11, 2002·Experimental and Molecular Pathology·K E MoyerH P Ehrlich

❮ Previous
Next ❯

Citations

May 6, 2006·Reproductive Biology and Endocrinology : RB&E·Ann Hjelm CluffGunvor Ekman-Ordeberg
Feb 13, 2016·Stem Cells·Russell M L SimpsonQingbo Xu
Feb 26, 2016·The Journal of Membrane Biology·Bruno CogliatiMathieu Vinken
Mar 14, 2008·Biology of Reproduction·Sagit Sela-AbramovichNava Dekel
Feb 16, 2018·Journal of the American Society of Nephrology : JASN·Ina Maria SchiesslHayo Castrop
Jun 10, 2004·Clinical Orthopaedics and Related Research·Andrew A MarinoJames A Albright
May 7, 2016·Cardiovascular Engineering and Technology·Ariel Greisas, Sharon Zlochiver
Jan 24, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ting GuoCongjian Xu
Dec 8, 2004·Cell Biochemistry and Function·H P EhrlichM Leggett

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.