Modulation of IgE-dependent COX-2 gene expression by reactive oxygen species in human neutrophils

Journal of Leukocyte Biology
Antonio VegaF Sobrino

Abstract

Cyclooxygenase (COX) is a key enzyme in prostaglandin (PG) synthesis. Up-regulation of its COX-2 isoform is responsible for the increased PG release, taking place under inflammatory conditions, and also, is thought to be involved in allergic and inflammatory diseases. In the present work, we demonstrate that COX-2 expression becomes highly induced by anti-immunoglobulin E (IgE) antibodies and by antigens in human neutrophils from allergic patients. This induction was detected at mRNA and protein levels and was accompanied by a concomitant PGE(2) and thromboxane A(2) release. We also show evidence that inhibitors of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, such as 4-(2-aminoethyl)benzenesulphonyl fluoride and 4-hydroxy-3-methoxyaceto-phenone, completely cancelled anti-IgE-induced COX-2 protein up-regulation, suggesting that this process is mediated by reactive oxygen species (ROS) derived from NADPH oxidase activity. Moreover, the mitogen-activated protein kinases (MAPKs), p38 and extracellular signal-regulated kinase, and also, the transcription factor, nuclear factor (NF)-kappaB, are involved in the up-regulation of COX-2 expression, as specific chemical inhibitors of these two kinases, such as SB20...Continue Reading

Citations

May 25, 2011·The Journal of Pharmacology and Experimental Therapeutics·Mohammed S El-AwadyS Jamal Mustafa
Jun 12, 2014·Kidney International·Shipra AgrawalWilliam E Smoyer
Apr 11, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Juanita MoraLarry L Thomas
Dec 20, 2007·Critical Care Medicine·Robert L ConhaimBruce A Harms
Oct 15, 2013·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Inmaculada VenturaJavier Monteseirín
Jun 30, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I GutowskaD Chlubek
Dec 4, 2015·Journal of Immunology Research·María A HidalgoRafael A Burgos
Oct 22, 2015·PloS One·Lourdes Fernández-DelgadoJavier Monteseirín
May 27, 2010·Human & Experimental Toxicology·François BinetDenis Girard
Jun 10, 2011·International Archives of Allergy and Immunology·Makiko FukunagaChisei Ra
Jun 19, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yan PiJing-Cheng Li
Apr 11, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lorena AlcañizJavier Monteseirin
Aug 31, 2006·The Journal of Biological Chemistry·Maria HatziapostolouEvangelia Papadimitriou
Apr 1, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pedro ChacónDavid Rivas-Pérez
Jun 29, 2021·Frontiers in Immunology·Raquel Freitas ZambonattoAdriana Cristina Levada-Pires

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.