Modulation of immune-associated surface markers and cytokine production by murine retinal glial cells

Journal of Neuroimmunology
K M Drescher, J A Whittum-Hudson

Abstract

Murine retinal glia are normally negative for major histocompatibility complex (MHC) Class II antigens and express low levels of MHC Class I and intercellular adhesion molecule-1 (ICAM-1) as detected by avidin-biotin-peroxidase immunohistochemistry. These surface molecules associated with immune function were either induced (Class II) or upregulated (Class I and ICAM-1) on cultured retinal glial cells in a dose- and time-dependent manner following exposure to recombinant interferon-gamma (rIFN-gamma). MHC Class I and II expression by passaged and primary cells was maximal (> 90% positive) after incubation with 100 U/m1 of rIFN-gamma for 48 h. ICAM-1 expression by primary and passaged cells tripled between 48 and 72 h after exposure to 25 or 50 U/m1 of rIFN-gamma. By 72 h after exposure to 100 U/m1 of rIFN-gamma, 62% of the retinal glia were positive for ICAM-1, whereas under normal culture conditions these molecules were detected on < 3% of the retinal glia. Bacterial lipopolysaccharide (LPS), a known stimulator of central nervous system (CNS) astrocytes, increased ICAM-1 expression only 3-fold to 9% of cells staining positively, but neither MHC Class I nor Class II expression was altered from baseline levels. Surface expressio...Continue Reading

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Citations

May 6, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Andreas Bringmann, Peter Wiedemann
Jun 20, 2013·Cell and Tissue Research·Roswitha SeitzErnst R Tamm
Aug 26, 2014·Mediators of Inflammation·Francisco J AscasoAndrzej Grzybowski
Feb 11, 2003·The Journal of Veterinary Medical Science·Tomohiro ImagawaMasato Uehara
Oct 21, 2006·FEMS Immunology and Medical Microbiology·Hervé C GérardAlan P Hudson
Jun 14, 2016·BioMed Research International·Rosa de HozJosé M Ramírez
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Aug 8, 2009·Progress in Retinal and Eye Research·Andreas BringmannAndreas Reichenbach

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