PMID: 6981046Jan 1, 1982Paper

Modulation of immune response by bacterial lipopolysaccharide (LPS): roles of macrophages and T cells in vitro adjuvant effect of LPS on antibody response to T cell-dependent and T cell-independent antigens

Microbiology and Immunology
T Uchiyama

Abstract

The roles of macrophages and T cells in the adjuvant effect of lipopolysaccharide (LPS) were studied. In vitro anti-trinitrophenyl (anti-TNP) antibody responses to TNP-Ficoll and TNP-keyhole limpet hemocyanine (TNP-KLH) in spleen cells in C57BL/6 mice showed the most enhancement, when LPS was added to cultures at 1 microgram/ml 48 hr after culture was started. The responses to these antigens were enhanced markedly by LPS in whole and macrophage-depleted spleen cells. The enhancement was greater in the latter group than in the former. The adjuvant effect among whole, T cell-depleted, macrophage-depleted and both macrophage- and T cell-depleted spleen cells was compared. The response to TNP-Ficoll was enhanced markedly by LPS in all groups. The enhancement was greater in the latter two groups than in the first two groups. The response to TNP-KLH was enhanced by LPS strongly in macrophage-depleted spleen cells, moderately in whole and both macrophage- and T cel-depleted spleen cells, and only slight in T cell-depleted spleen cells. Enhancement was restored to T cell-depleted spleen cells by adding T cells. The response to TNP-KLH of macrophage-depleted spleen cells of LPS-responsive C3H/HeN mice which was enhanced by LPS was suppr...Continue Reading

References

Dec 1, 1977·The Journal of Experimental Medicine·M K HoffmannH F Oettgen
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Aug 1, 1974·Journal of Immunological Methods·I A Ly, R I Mishell
Nov 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·M B Rittenberg, K L Pratt

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Citations

Feb 22, 2008·Fish & Shellfish Immunology·S K NayakN K Maiti
Feb 19, 2014·Journal of Dairy Science·R ZanabriaM Corredig

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