Modulation of integrin-linked kinase (ILK) expression in human oesophageal squamous cell carcinoma cell lines by the EGF and TGFbeta1 growth factors.

Cancer Cell International
Glenn A Driver, Robin B Veale

Abstract

Integrin-linked kinase (ILK) is a ubiquitously expressed protein kinase that has emerged as one of the points of convergence between integrin- and growth factor-signalling pathways. In this study we identify the ILK isoform expressed in five human oesophageal squamous cell carcinoma cell lines of South African origin as ILK1, and demonstrate its cellular distribution. ILK expression, although similar in the majority of the cell lines, did show variation. Furthermore, the ILK expressed was shown to be catalytically functional. The effect of growth factors on ILK expression was examined. An increase in ILK expression, following EGF and TGFbeta1 exposure, was a trend across all the five oesophageal carcinoma cell lines tested. These results suggest that growth factor modulation of ILK expression relies on the internalisation/recycling of growth factor receptors and stimulation of the PI3K pathway, which may have implications with regards to cell adhesion and tumourigenesis.

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Citations

Mar 21, 2017·Nitric Oxide : Biology and Chemistry·Emanuela FerrettiJean-François Beaulieu
Jul 14, 2009·Experimental Cell Research·Raghvendra SinghStelios T Andreadis
May 1, 2010·Journal of Cellular Physiology·James BeattieChristopher F van der Walle
Sep 25, 2008·Virchows Archiv : an International Journal of Pathology·Anastasios K GoulioumisHelen Papadaki

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Methods Mentioned

BETA
electrophoresis
PCR
restriction digests
immunoprecipitation

Software Mentioned

[UNK] Image Acquisition and Analysis

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