PMID: 8581744Sep 1, 1995Paper

Modulation of interleukin-1 receptor expression by transforming growth factor-beta in cultured rabbit articular chondrocytes: analysis by reverse transcription-polymerase chain reaction

Osteoarthritis and Cartilage
S PronostJ P Pujol

Abstract

Interleukin-1 receptor type I (IL-1RI) expression in cultured rabbit articular chondrocytes (RAC) was studied by reverse transcription-polymerase chain reaction (RT-PCR). A cDNA probe specific for the rabbit IL-1RI gene was constructed using primers derived from the sequence data of the human, murine and chick receptors. Transforming growth factor-beta 1 (TGF beta-1) was shown to transiently increase the level of expected 900-bp PCR product at 1 h of incubation and decrease the expression at 48 and 72 h with no effect at 24 h. In receptor binding assays using [125I]-IL-1 alpha, TGF beta decreased IL-1R bioactivity at all time points. These results suggest that TGF beta-induced down-regulation of IL-1 RI could be responsible for its ability to antagonize the effect of IL-1 and that TGF beta may have a role in the repair of articular cartilage.

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Citations

Mar 30, 2007·Osteoarthritis and Cartilage·E N Blaney DavidsonW B van den Berg
Jan 15, 2014·Osteoarthritis and Cartilage·R ParekhA L Clark
Aug 28, 2019·Cells·Nathalie G M ThielenArjan P M van Caam
Nov 9, 2000·Osteoarthritis and Cartilage·Y E HenrotinJ Y Reginster

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