PMID: 11329616May 1, 2001Paper

Modulation of intestinal and liver fatty acid-binding proteins in Caco-2 cells by lipids, hormones and cytokines

Journal of Cellular Biochemistry
N DubéE Levy

Abstract

Intestinal and liver fatty acid binding proteins (I- and L-FABP) are thought to play a role in enterocyte fatty acid (FA) trafficking. Their modulation by cell differentiation and various potential effectors was investigated in the human Caco-2 cell line. With the acquisition of enterocytic features, Caco-2 cells seeded on plastic progressively increased L-FABP quantities, whereas I-FABP was not detectable even very late in the maturation process. On permeable filters that improved differentiation markers (sucrase, alkaline phosphatase, transepithelial resistance), Caco-2 cells furthered their L-FABP content and expressed I-FABP. Western blot analysis showed a significant increase in I- and L-FABP expression following an 8-hour incubation period with butyric acid, oleic acid, and phosphatidylcholine. However, in all cases, I-FABP levels were higher than L-FABP concentrations regardless of the lipid substrates added. Similarly, hydrocortisone and insulin enhanced the cellular content of I- and L-FABP whereas leptin triggered I-FABP expression only after an 8-hour incubation. Finally, tumor necrosis factor-alpha was more effective in increasing the cytosolic amount of I-FABP levels. In conclusion, our data demonstrate that I-FABP...Continue Reading

References

Dec 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·K Adeli, A Theriault
Nov 1, 1992·Pediatric Research·D D Black, H Ellinas
Dec 15, 1992·Biochimica Et Biophysica Acta·N LoirdighiE Levy
Jun 30, 1986·Biochemical and Biophysical Research Communications·N M Bass, J A Manning
Nov 1, 1983·Biochimica Et Biophysica Acta·Y KawashimaH Kozuka
Jan 1, 1995·Progress in Lipid Research·J H Veerkamp, R G Maatman
Feb 1, 1995·Journal of Pediatric Gastroenterology and Nutrition·D D Black
May 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E LevyE Seidman
Dec 1, 1995·The American Journal of Physiology·M MehranE Levy
Feb 1, 1997·In Vitro Cellular & Developmental Biology. Animal·M MehranE Seidman
Jan 1, 1997·Annual Review of Nutrition·D A BernlohrL J Banaszak
Sep 25, 1998·The Journal of Biological Chemistry·N M MortonM A Cawthorne

❮ Previous
Next ❯

Citations

Jul 3, 2007·Histochemistry and Cell Biology·S GuilmeauM E Samson-Bouma
Jun 6, 2009·Histochemistry and Cell Biology·Emile LevyMoise Bendayan
Nov 1, 2002·General and Comparative Endocrinology·Richard L Londraville, Charles S Duvall
Nov 27, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Nan ZuoYasuhiko Tomino
Jul 26, 2008·Atherosclerosis. Supplements·Joanne HsiehKhosrow Adeli
Mar 9, 2007·Biochemical and Biophysical Research Communications·Maja KlapperFrank Döring
Nov 22, 2008·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·A J O'Sullivan
Mar 29, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Margarita VidaElena Baixeras
Jan 1, 2012·Archives of Insect Biochemistry and Physiology·Zhiqiang HuangLin Liu
Apr 1, 2015·Journal of Cellular Biochemistry·Alain SanéEmile Levy
Jul 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Philippe G CammisottoMoïse Bendayan
Mar 3, 2007·Nature Reviews. Drug Discovery·Christopher J H PorterWilliam N Charman
Jul 12, 2007·The Journal of Pharmacology and Experimental Therapeutics·Patrick HindletMarion Buyse
Jan 23, 2009·Molecular and Cellular Biochemistry·Tony VelkovMartin J Scanlon
Jun 27, 2020·Progress in Lipid Research·Rainer H Straub
Oct 7, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Alain Théophile SanéEmile Levy
Jan 27, 2005·Gastroentérologie Clinique Et Biologique·Valérie MarcilEmile Levy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.