Modulation of intracellular Ca2+ concentration in brain microvascular endothelial cells in vitro by acoustic cavitation.

Ultrasound in Medicine & Biology
Juyoung ParkCheri X Deng

Abstract

Localized delivery of therapeutic agents through the blood-brain barrier (BBB) is a clinically significant task that remains challenging. Ultrasound (US) application after intravenous administration of microbubbles has been shown to generate localized BBB opening in animal models but the detailed mechanisms are not yet fully described. The current study investigates the effects of US-stimulated microbubbles on in vitro murine brain microvascular endothelial (bEnd.3) cells by monitoring sonoporation and changes in intracellular calcium concentration ([Ca(2+)](i)) using real-time fluorescence and high-speed brightfield microscopy. Cells seeded in microchannels were exposed to a single US pulse (1.25 MHz, 10 cycles, 0.24 MPa peak negative pressure) in the presence of Definity microbubbles and extracellular calcium concentration [Ca(2+)](o) = 0.9 mM. Disruption of the cell membrane was assessed using propidium iodide (PI) and change in the [Ca(2+)](i) was measured using fura-2. Cells adjacent to a microbubble exhibited immediate [Ca(2+)](i) changes after US pulse with and without PI uptake and the [Ca(2+)](i) changes were twice as large in cells with PI uptake. Cell viability assays showed that sonoporated cells could survive with ...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S K NigamR B Silver
Jan 1, 1986·Journal of Applied Physiology·D M Shasby, S S Shasby
Jun 1, 1983·Experimental Neurology·B T StokesG Hollinden
Feb 22, 1994·The Journal of Comparative Neurology·P A Stewart, U I Tuor
Jan 14, 1994·Brain Research. Developmental Brain Research·M Puka-SundvallP Andiné
Jan 1, 1994·Molecular and Cellular Endocrinology·M J SandersonE R Dirksen
Jun 1, 1994·Journal of Cellular Physiology·R O StuartS K Nigam
Jul 1, 1993·Experimental Physiology·M W Bradbury
Dec 1, 1996·Journal of Neurosurgery·B ZünkelerE H Oldfield
Jun 27, 1998·The Journal of Neuroscience Nursing : Journal of the American Association of Neuroscience Nurses·N D DoolittleS Eriksen
Mar 4, 2000·Cellular and Molecular Neurobiology·N J Abbott
Jun 21, 2001·Echocardiography·K Tachibana, S Tachibana
Feb 22, 2002·Medicinal Research Reviews·Ka-yun Ng, Yang Liu
Apr 5, 2002·The Journal of the Acoustical Society of America·Joel P RossJunru Wu
Jun 8, 2002·Stroke; a Journal of Cerebral Circulation·Rachel C Brown, Thomas P Davis
Aug 31, 2002·Endothelium : Journal of Endothelial Cell Research·Shailesh Y DesaiDamir Janigro
Nov 21, 2002·Neuron·William M Pardridge
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
May 4, 2004·Ultrasound in Medicine & Biology·Cheri X DengJianmin Cui
Jun 9, 2004·Ultrasound in Medicine & Biology·Hidemi HondaHiroshi Kitagawa
Aug 18, 2004·Ultrasound in Medicine & Biology·Nickolai SheikovKullervo Hynynen
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·William M Pardridge
Dec 24, 2005·Nature Reviews. Neuroscience·N Joan AbbottElisabeth Hansson
Jan 13, 2006·Biochemical and Biophysical Research Communications·Manabu KinoshitaKullervo Hynynen
Apr 25, 2006·American Journal of Physiology. Heart and Circulatory Physiology·L J M JuffermansO Kamp
Jun 21, 2006·Ultrasound in Medicine & Biology·Robyn K SchlicherMark R Prausnitz
Jul 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Manabu KinoshitaKullervo Hynynen
Dec 26, 2006·Ultrasound in Medicine & Biology·James J ChoiElisa E Konofagou
Dec 26, 2006·Ultrasound in Medicine & Biology·T A TranA Bouakaz

❮ Previous
Next ❯

Citations

Jan 28, 2014·Advanced Drug Delivery Reviews·Muna AryalNathan McDannold
May 27, 2014·Therapeutic Delivery·Zhenzhen FanCheri X Deng
Oct 21, 2010·Molecular Pharmaceutics·Stephanie J GraingerMohamed E H El-Sayed
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhenzhen FanCheri X Deng
Feb 26, 2016·Neuropharmacology·Charissa PoonKullervo Hynynen
Sep 26, 2013·Ultrasound in Medicine & Biology·Yaxin HuAlfred C H Yu
Jun 20, 2013·Journal of Healthcare Engineering·Yufeng Zhou
Oct 29, 2011·Ultrasound in Medicine & Biology·Wenjing ZhongAlfred C H Yu
Dec 30, 2014·IEEE Transactions on Medical Imaging·Costas D ArvanitisNathan McDannold
Apr 7, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·S RizzitelliE Terreno
Jan 28, 2014·Ultrasound in Medicine & Biology·Peng QinAlfred C H Yu
Mar 19, 2013·Ultrasound in Medicine & Biology·Xian ChenAlfred C H Yu
May 29, 2016·Pharmacology & Therapeutics·Angeliki Tiptiri-KourpetiKaterina Chlichlia
Apr 5, 2017·Biomedical Engineering Letters·Mun HanJuyoung Park
Jan 20, 2021·Experimental Biology and Medicine·Madhumithra Subramanian Karthikesh, Xinmai Yang
Dec 13, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Eric K JuangMichalakis Averkiou
Apr 26, 2020·Ultrasound in Medicine & Biology·Antoine PressetNoboru Sasaki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.