PMID: 9168468May 1, 1997Paper

Modulation of intracellular calcium levels inhibits secretion of collagenase 1 by migrating keratinocytes

Molecular Biology of the Cell
B D SudbeckW C Parks

Abstract

Calcium concentration influences keratinocyte differentiation, and, following injury, keratinocytes move through an environment of changing calcium levels. Because these migrating cells in wounds invariably express collagenase 1, we assessed if modulation of calcium levels regulates collagenase 1 production by primary human keratinocytes. Accurately reflecting the confined spatial pattern of enzyme production seen in vivo, collagenase 1 mRNA was expressed only by keratinocytes migrating from foci of differentiated cells. Treatment with calcium ionophores A23187 or thapsigargin markedly inhibited the basal and phorbol 12-myristate 13-acetate-(PMA) stimulated accumulation of keratinocyte collagenase 1 in the medium but did not affect collagenase 1 production by control or PMA-treated fibroblasts. A23187-mediated inhibition of collagenase 1 protein was not associated with a decrease in mRNA levels but rather was controlled by a selective and reversible block of enzyme secretion. This block in secretion was likely not due to altered protein folding as the proenzyme within A23187-treated cells remained capable of autolytic activation upon treatment with p-aminophenylmercuric acetate. In contrast, 92-kDa gelatinase mRNA and secreted ...Continue Reading

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Citations

Dec 14, 2011·Journal of Dermatological Science·Young Mee LeeJin Ho Chung
Dec 20, 2013·The Journal of Investigative Dermatology·Maryam G RohaniWilliam C Parks
Nov 9, 2000·The Journal of Investigative Dermatology·O RechardtU Saarialho-Kere
Jun 15, 2007·Journal of Cellular Physiology·Edel A O'TooleDavid T Woodley
Nov 20, 2010·Molecular Nutrition & Food Research·Yong Pil HwangHye Gwang Jeong
Aug 10, 1999·Experimental Cell Research·M MäkeläV J Uitto
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B SteffensenH Larjava
Apr 29, 1998·Annual Review of Physiology·B Capel
Sep 3, 1999·The Journal of Biological Chemistry·G T Park, M I Morasso
Oct 16, 1999·The Journal of Biological Chemistry·P Sorensen, E Wintersberger
Oct 15, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shi-Meng ZhangPing-Kun Zhou
Jun 14, 2002·Biochimica Et Biophysica Acta·Candice TrocméFrançoise Morel

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