PMID: 15228091Jul 2, 2004Paper

Modulation of intracellular calcium concentrations and T cell activation by prickly pear polyphenols

Molecular and Cellular Biochemistry
Virginie AiresN A Khan

Abstract

Opuntia ficus indica (prickly pear) polyphenolic compounds (OFPC) triggered an increase in [Ca2+]i in human Jurkat T-cell lines. Furthermore, OFPC-induced rise in [Ca2+]i was significantly curtailed in calcium-free buffer (0% Ca2+) as compared to that in 100% Ca2+ medium. Preincubation of cells with tyrphostin A9, an inhibitor of Ca2+ release-activated Ca2+ (CRAC) channels, significantly diminished the OFPC-induced sustained response on the increases in [Ca2+]i. Lanthanum and nifedipine, the respective inhibitors of voltage-dependent and L-type calcium channels, failed to curtail significantly the OFPC-induced calcium response. As OFPC still stimulated increases in [Ca2+]i in 0% Ca2+ medium, the role of intracellular calcium was investigated. Hence, addition of thapsigargin (TG), an inhibitor of Ca2+-ATPase of the endoplasmic reticulum (ER), during the OFPC-induced peak response exerted an additive effect, indicating that the mechanism of action of these two agents are different. Furthermore, U73122, an inhibitor of IP3 production, completely abolished increases in [Ca2+]i, induced by OFPC, suggesting that these polyphenols induce the production of IP3 that recruits calcium from ER pool. Polyphenolic compounds do act extracellu...Continue Reading

Citations

Sep 28, 2010·BMC Complementary and Alternative Medicine·Chahid BenammarNaim A Khan
Dec 3, 2015·BMC Complementary and Alternative Medicine·Abdelhafid NaniNaim Akhtar Khan
Jun 3, 2015·Pakistan Journal of Biological Sciences : PJBS·Nizar YeddesMalika Trabelsi Ayadi
Jun 1, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A SinghA Mercenier
Sep 3, 2008·Journal of Zhejiang University. Science. B·Rui-wei Guo, Lan Huang
Mar 7, 2021·Nutrients·Hira ShakoorLily Stojanovska

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