PMID: 11909640Mar 23, 2002Paper

Modulation of intrinsic P-glycoprotein expression in multicellular prostate tumor spheroids by cell cycle inhibitors

Biochimica Et Biophysica Acta
M WartenbergH Sauer

Abstract

The effects of cell cycle inhibition on the expression of the multidrug resistance transporter P-glycoprotein (P-gp) as well as of the cyclin-dependent kinase (CDK) inhibitors p27(Kip1) and p21(WAF-1) were investigated in DU-145 prostate tumor spheroids. With increasing spheroid size the number of cells in the G0/G1 phase augmented, whereas the number of cells in the G2/M phase and the S phase of the cell cycle declined. The number of G0/G1 cells was elevated after incubation with either mimosine, staurosporine or serum-free medium. Mitomycin C and roscovitine increased the number of S phase cells. Roscovitine additionally increased cells in the G2/M phase. Incubation in serum-free medium upregulated p21(WAF-1), p27(Kip1) and P-gp. Mimosine treatment resulted in upregulation of p27(Kip1) and P-gp, whereas p21(WAF-1) remained unchanged. Upon roscovitine treatment p27(Kip1) and p21(WAF-1) were downregulated, whereas P-gp was unaltered. Mitomycin C treatment resulted in downregulation of p27(Kip1) and p21(WAF-1); no significant change in P-gp levels was observed. Staurosporine induced upregulation of p21(WAF-1) whereas p27(Kip1) remained unaltered. P-gp was downregulated upon staurosporine treatment, which was owing to an elevatio...Continue Reading

Citations

Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Maurizio ScaltritiJosé Baselga
Sep 15, 2004·Tissue Engineering·Hong SongKim C O'Connor
Sep 16, 2003·Molecular Biology of the Cell·Zizheng Dong, Jian-Ting Zhang
Jul 16, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Can SarisozenVladimir P Torchilin
Dec 7, 2007·International Journal of Radiation Biology·Juergen FriedrichLeoni A Kunz-Schughart
Apr 25, 2015·Drug Discovery Today·Viswanath DasMarián Hajdúch
Feb 28, 2013·Expert Opinion on Drug Delivery·Abraham H Abouzeid, Vladimir P Torchilin
Jul 30, 2015·Cancer Cell International·Raúl Barrera-Rodríguez, Jorge Morales Fuentes
May 10, 2015·International Immunopharmacology·Xian-Wei LiJie-Ren Yang
Oct 14, 2005·World Journal of Gastroenterology : WJG·Min-Wen HaYuan Yuan
Jun 17, 2020·Experimental Hematology & Oncology·Jingjing WuJen Wei Chiao
Jan 13, 2004·World Journal of Gastroenterology : WJG·Min-Wen HaYuan Yuan
Sep 20, 2020·International Journal of Molecular Sciences·Fabrizio FontanaPatrizia Limonta

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