Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench)

Plant Biotechnology Journal
Tejinder KumarTom Clemente

Abstract

Sorghum prolamins, termed kafirins, are categorized into subgroups α, β, and γ. The kafirins are co-translationally translocated to the endoplasmic reticulum (ER) where they are assembled into discrete protein bodies that tend to be poorly digestible with low functionality in food and feed applications. As a means to address the issues surrounding functionality and digestibility in sorghum, we employed a biotechnology approach that is designed to alter protein body structure, with the concomitant synthesis of a co-protein in the endosperm fraction of the grain. Wherein perturbation of protein body architecture may provide a route to impact digestibility by reducing disulphide bonds about the periphery of the body, while synthesis of a co-protein, with known functionality attributes, theoretically could impact structure of the protein body through direct association and/or augment end-use applications of sorghum flour by stabilizing ß-sheet formation of the kafirins in sorghum dough preparations. This in turn may improve viscoelasticity of sorghum dough. To this end, we report here on the molecular and phenotypic characterizations of transgenic sorghum events that are down-regulated in γ- and the 29-kDa α-kafirins and the expres...Continue Reading

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Citations

Oct 24, 2013·Journal of Agricultural and Food Chemistry·Joseph O AnyangoJanet Taylor
Oct 6, 2015·Journal of Proteomics·André Luis Wendt Dos SantosEny Iochevet Segal Floh
Oct 30, 2016·Critical Reviews in Food Science and Nutrition·Leandro de Morais CardosoHelena Maria Pinheiro-Sant'Ana
Jul 8, 2014·Frontiers in Plant Science·David R Holding
Oct 14, 2021·Journal of Experimental Botany·Tallyta N SilvaJenny C Mortimer

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