Modulation of laccase transcriptome during biodegradation of naphthalene by white rot fungus Pleurotus ostreatus

International Microbiology : the Official Journal of the Spanish Society for Microbiology
Shaza M ElhusseinyRania I Shebl

Abstract

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) using Pleurotus ostreatus was investigated in the current study along with the expression levels of laccase genes involved in biodegradation under variable conditions. Biodegradation of PAHs (naphthalene, anthracene, and 1,10-phenanthroline) was detected spectrophotometrically. Recorded data revealed that biodegradation of the tested PAHs was time dependent. Elevated level of naphthalene biodegradation (86.47%) was observed compared to anthracene (27.87%) and 1,10-phenanthroline (24.51%) within 3 days post incubation. Naphthalene was completely degraded within 5 days. Further incubation enhanced the biodegradation of both anthracene and 1,10-phenanthroline until reaches 93.69% and 92.00% biodegradation of the initial concentration within an incubation period of 11 and 14 days, respectively. Naphthalene was selected as a PAH model. HPLC and thin layer chromatography of naphthalene biodegradation products at time intervals proposed that naphthalene was first degraded to α- and β-naphthol which was further metabolized to salicylic and benzoic acid. The metabolic pathway of naphthalene degradation by this fungus was elucidated based on the detected metabolites. The expressio...Continue Reading

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Citations

Jul 16, 2020·Environmental Microbiology·Heidy Peidro-GuzmánRamón Alberto Batista-García
Aug 24, 2021·Fungal Biology·Farzaneh RoshandelZahra Oraghi Ardebili

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