Modulation of lactate dehydrogenase activity by enzyme-protein interaction

Journal of Enzyme Inhibition
M L SagristáC Lluis

Abstract

Some lactate dehydrogenase modulator proteins have been isolated from the lactate dehydrogenase-free crude mitochondrial fraction of rabbit muscle, beef liver and chicken liver. It was shown that beef and chicken liver mitochondrial extracts exhibited activatory capacity in contrast to the inhibitory capacity of rabbit muscle mitochondrial extracts. All modulators can be precipitated by 80% ammonium sulphate saturation and show high anodic electrophoretic mobility and heat stability. Modulators have higher affinity for alkaline pI lactate dehydrogenase isoenzymes, independent of whether the M and H subunits are predominant. The inhibitor and the activator molecules compete for lactate dehydrogenase since their modulatory capacity was nullified when similar relative amounts were used. This study shows the existence of analogous proteins with an acidic pI in the different mitochondrial fractions which modify lactate dehydrogenase activity.

References

Jan 13, 1975·Biochimica Et Biophysica Acta·F M Clarke, C J Masters
Mar 1, 1986·Archives of Biochemistry and Biophysics·R MarquetantE W Holmes
Sep 1, 1972·Archives of Biochemistry and Biophysics·D N Skilleter, E Kun
Jan 1, 1973·Archives of Biochemistry and Biophysics·J D Ehmann, H O Hultin
Jan 1, 1984·The International Journal of Biochemistry·W L ElliottP F Heinstein
Jan 1, 1980·Journal of Neurochemistry·H R KnullW G Niehaus
Jan 1, 1981·CRC Critical Reviews in Biochemistry·C J Masters

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Citations

Apr 1, 1994·The Histochemical Journal·Y Nakae, P J Stoward

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