Modulation of Light Energy Transfer from Chromophore to Protein in the Channelrhodopsin ReaChR.

Biophysical Journal
Joel C D KaufmannFranz J Bartl

Abstract

The function of photoreceptors relies on efficient transfer of absorbed light energy from the chromophore to the protein to drive conformational changes that ultimately generate an output signal. In retinal-binding proteins, mainly two mechanisms exist to store the photon energy after photoisomerization: 1) conformational distortion of the prosthetic group retinal, and 2) charge separation between the protonated retinal Schiff base (RSBH+) and its counterion complex. Accordingly, energy transfer to the protein is achieved by chromophore relaxation and/or reduction of the charge separation in the RSBH+-counterion complex. Combining FTIR and UV-Vis spectroscopy along with molecular dynamics simulations, we show here for the widely used, red-activatable Volvox carteri channelrhodopsin-1 derivate ReaChR that energy storage and transfer into the protein depends on the protonation state of glutamic acid E163 (Ci1), one of the counterions of the RSBH+. Ci1 retains a pKa of 7.6 so that both its protonated and deprotonated forms equilibrate at physiological conditions. Protonation of Ci1 leads to a rigid hydrogen-bonding network in the active-site region. This stabilizes the distorted conformation of the retinal after photoactivation an...Continue Reading

References

Jun 28, 1977·Biochemistry·B AtonT G Ebrey
Dec 2, 1975·Biochemistry·R H AustinI C Gunsalus
Mar 1, 1988·Biophysical Journal·R R BirgeL P Murray
Apr 1, 1983·Biophysical Journal·R R Birge, T M Cooper
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Jun 29, 2002·Science·Georg NagelPeter Hegemann
Aug 31, 2002·Biophysical Journal·Shigehiko HayashiKlaus Schulten
Oct 30, 2003·Journal of the American Chemical Society·Mikihiro ShibataHideki Kandori
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Sep 2, 2004·Biophysical Journal·Jose A Gascon, Victor S Batista
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrei K Dioumaev, Janos K Lanyi
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Nov 27, 2007·Journal of Molecular Biology·Christian BamannErnst Bamberg
Mar 21, 2008·Journal of Computational Chemistry·Sunhwan JoWonpil Im
Feb 5, 2009·Photochemistry and Photobiology·Satoshi P Tsunoda, Peter Hegemann
Jan 19, 2010·Nature Neuroscience·Lisa A GunaydinPeter Hegemann
Feb 4, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Melanie NackJoachim Heberle
May 26, 2010·The Journal of Physical Chemistry. B·Jeffery B KlaudaRichard W Pastor
Mar 12, 2011·Journal of the American Chemical Society·Sivakumar Sekharan, Keiji Morokuma
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Jan 24, 2012·Nature·Hideaki E KatoOsamu Nureki
Jan 1, 2009·Sensors·Akira Kawanabe, Hideki Kandori
Mar 30, 2013·Journal of the American Chemical Society·Mirka-Kristin Neumann-VerhoefenJosef Wachtveitl
Sep 3, 2013·The Journal of Biological Chemistry·Elena G GovorunovaJohn L Spudich
Feb 12, 2014·Journal of the American Chemical Society·Shota ItoHideki Kandori
Apr 18, 2014·Biophysical Journal·Hai LiJohn L Spudich
May 2, 2014·Nucleic Acids Research·Marco BiasiniTorsten Schwede

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Citations

Oct 15, 2021·Communications Biology·Yuta NakajimaHideki Kandori

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