Modulation of lipid polymorphism by the feline leukemia virus fusion peptide: implications for the fusion mechanism

Biochemistry
S M DaviesR F Epand

Abstract

The structural effects of the fusion peptide of feline leukemia virus (FeLV) on lipid polymorphism were studied, using differential scanning calorimetry (DSC), 31P nuclear magnetic resonance (NMR), and time-resolved X-ray diffraction. This peptide lowers the bilayer to inverted hexagonal phase transition temperature, TH, of dipalmitoleoylphosphatidylethanolamine (DiPoPE) at peptide mole fractions of up to 1.5 x 10(-3) at pH 5.0 and at pH 7.4. The temperature at which isotropic 31P NMR signals for monomethyldioleoylphosphatidylethanolamine (MeDOPE) first occurred is lowered by the FeLV peptide. The amount of isotropic signal seen at 40 degrees C is directly correlated to the peptide:lipid molar ratio. In the peptide-containing samples, more lipid remains in the isotropic state over the whole recorded temperature range. Isotropic 31P NMR signals were observed for DiPoPE in the presence of the FeLV peptide for the entire recorded temperature range of 35-50 degrees C, while pure DiPoPE showed no significant amount of isotropic signal. X-ray studies of DiPoPE show the formation of a new lipid phase with peptide, which is not seen in the pure lipid samples. Disordering of the Lalpha phase is evidenced by broadening of the diffraction...Continue Reading

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Citations

Mar 10, 2001·Biopolymers·R M Epand, R F Epand
May 1, 2005·Journal of Biological Physics·Thad A HarrounJeremy P Bradshaw
Nov 23, 2005·Biochemistry·Jon-Paul S PowersRobert E W Hancock
Jun 29, 2000·Annual Review of Biochemistry·R Jahn, T C Südhof
May 20, 2014·Biochimica Et Biophysica Acta·Richard M EpandMichael Schlame
Jul 27, 2010·Journal of Molecular Biology·Stephanie Tristram-NagleJohn F Nagle
Jan 22, 2008·Experimental and Molecular Pathology·Raquel F EpandYanina Rozenberg-Adler
Jul 23, 2003·Biochimica Et Biophysica Acta·José L Nieva, Aitziber Agirre
Jan 3, 2015·Biochimica Et Biophysica Acta·Carmen L DelgadoFrancisco Gavilanes
Jul 15, 2015·Biophysical Journal·Mariya ChavarhaStephen B Hall
Sep 23, 2008·Biophysical Journal·David P Siegel
May 4, 2002·Biochimica Et Biophysica Acta·Malcolm J M DarkesJeremy P Bradshaw
Aug 5, 2014·Chemistry and Physics of Lipids·Marc FuhrmansMarcus Müller

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