Modulation of liver-specific cellular response to ethanol in vitro in hep G2 cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
R G CameronC Tiribelli

Abstract

The aim of this study was to investigate in vitro in a human hepatoblastoma cell line, Hep G2, the effect of ethanol (EtOH) toxicity. The ultrastructural changes were assessed by performing quantitative light and transmission electron microscopy. The second objective of this study was to define further EtOH-induced biochemical changes associated with mitochondrial function. In comparison with controls, after exposure to 80 mm EtOH cells showed: a threefold increase in length of mitochondria; proliferation, vesiculation and dilatation of smooth endoplasmic reticulum, and twofold increases in the size of lipid droplets and in their number/cell. Exposure of cells to two doses of EtOH augmented the ultrastructural alterations observed after a single dose. Cytoviability, assessed by metabolism of methylxanthine dye decreased significantly by (P < 0.0001) to 68% of the control after one dose and was further reduced after the second dose of EtOH (P < 0.001). Succinate dehydrogenase activity in cells treated for 24 hr with 80 mm EtOH was decreased to about 80% of control values after one 24-hr treatment with 80 mM EtOH and to about 55% of control values after two 24-hr exposures. This in vitro model of ethanol-induced cytotoxicity in H...Continue Reading

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Citations

Dec 22, 2000·Clinical Biochemistry·M G NeumanN H Shear
Jun 16, 2001·Clinical Biochemistry·M G NeumanF Abbott
Jun 16, 2001·Clinical Biochemistry·G G KatzM G Neuman
Apr 9, 2002·Clinical Biochemistry·Alpa PopatManuela G Neuman
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Dec 30, 1999·Clinical Biochemistry·M G NeumanC Tiribelli
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