Modulation of lymphocyte proliferation by enzymes that degrade amino acids

Clinical and Experimental Immunology
J C ChuangS R Wang

Abstract

In a previous study we demonstrated thirteen amino acids to be essential and two to be partially essential for lymphocyte proliferation. Arginine is one of the essential amino acids, and the highly purified arginase strongly inhibited lymphocyte proliferation. The modulation of lymphocyte growth by various amino acid-degrading enzymes was studied. Peripheral lymphocytes were cultured in RPMI 1640 with or without amino acid-degrading enzyme for 72 h. A total of 17 commercial L-amino acid-degrading enzymes were studied. At 10 micrograms/ml, both lysine decarboxylase and asparaginase completely inhibited lymphocyte proliferation, arginase resulted in 78% inhibition and tyrosinase 57% inhibition. Other enzymes inhibited less than 20% lymphocyte proliferation; they included alanine dehydrogenase, arginine decarboxylase, aspartase, glutamic decarboxylase, glutamic dehydrogenase, glutaminase, histidase, histidine decarboxylase, leucine dehydrogenase, phenylalanine decarboxylase, phenylalanine hydroxylase, tryptophanase, and tyrosine decarboxylase. All four enzymes that strongly inhibited lymphocyte proliferation degraded amino acids that are essential for lymphocyte growth.

References

Mar 1, 1978·Cellular Immunology·S R Wang, B Zweiman
Jul 1, 1990·Clinical and Experimental Immunology·J C ChuangS R Wang
Feb 22, 1969·Lancet·G AstaldiA A Astaldi
Apr 5, 1968·Biochemical and Biophysical Research Communications·M D Prager, N Bachynsky
Feb 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·M S Simberkoff, L Thomas
May 3, 1968·Science·B HorowitzE Stockert

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Citations

Oct 26, 2016·Current Diabetes Reports·Anne Julie OvergaardFlemming Pociot
May 2, 2017·American Journal of Respiratory Cell and Molecular Biology·Ma'en ObeidatPeter D Paré
Feb 24, 2001·JPEN. Journal of Parenteral and Enteral Nutrition·J B OchoaE Fitzpatrick
Jun 6, 2020·Frontiers in Immunology·Tomasz M GrzywaJakub Golab
Oct 1, 2015·Pediatric Diabetes·Brigitte I Frohnert, Marian J Rewers
Mar 13, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·D M Goldberg

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