Modulation of MCF-7 cell proliferative responses by manipulation of assay conditions

Toxicology in Vitro : an International Journal Published in Association with BIBRA
P A JonesL K Earl

Abstract

The use of MCF-7 cell proliferation assays has been proposed as one of a number of available in vitro assays to determine the oestrogenic potential of chemicals. However, there is concern that differences between strains of MCF-7 cells and protocols used may lead to significant interlaboratory variability, particularly in their response to the natural oestrogen 17beta-oestradiol, and inability to detect weak oestrogens (Villalobos et al., 1995). This investigation adds to the debate by demonstrating that manipulation of assay conditions may modulate the proliferative response of a particular MCF-7 cell strain to 17beta-oestradiol. MCF-7 cells were put in 12-well plates, then exposed for 6 days to 17)3-oestradiol in EMEM without phenol red using serum stripped of endogenous oestrogens (EMEM/H-). A maximum proliferation of 200-250% control (assessed by fluorescein diacetate uptake) was observed, which is lower than some reported values (up to eightfold increase) and would probably reduce the sensitivity of detection of a weak oestrogen. Preincubation of cells in EMEM/H- for 48 hr, prior to the addition of 17beta-estradiol, resulted in an increased maximum proliferation of 400% control or more. Differences were also seen in the sh...Continue Reading

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Citations

May 12, 2000·The Science of the Total Environment·J PayneA Kortenkamp
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V A Baker
Jun 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J OdumJ Ashby
Aug 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P A JonesL K Earl
Nov 1, 2002·Breast Cancer Research and Treatment·Cornelia Bodinet, Johannes Freudenstein
Oct 25, 2002·Journal of Toxicology and Environmental Health. Part a·Haiyan ChenXinru Wang
May 1, 2004·The Journal of Reproduction and Development·In Young KimSoon Young Han
Mar 10, 2016·Biochemistry Research International·Colin P FlorianJennifer R Schroeder
May 29, 2004·Menopause : the Journal of the North American Menopause Society·Cornelia Bodinet, Johannes Freudenstein

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