Modulation of membrane conductance in rods of Bufo marinus by intracellular calcium ion

The Journal of Physiology
B Oakley, L H Pinto

Abstract

Double-barrel micropipettes were used to pressure-inject EGTA into the outer segments of rods in the isolated retina of Bufo marinus. We used these pipettes to point voltage clamp the cell to its resting membrane voltage during the injection of EGTA in order to prevent changes in membrane voltage from occurring. The input conductance of the rod was assessed by measuring the incremental membrane current required to hyperpolarize the membrane by less than or equal to 10 mV. When the retina was bathed in normal Ringer solution, the injection of EGTA during point voltage clamp evoked an inward membrane current and in increase in input conductance. This observation is consistent with an EGTA-evoked increase in conductance for an ion with an equilibrium potential more depolarized than the resting membrane potential. Injections of control solutions that did not contain EGTA had no effect. The effects of injected EGTA were not altered by variations in the pH or buffering capacity of the injection solution, or by the addition of equimolar Mg2+. Furthermore, injections of a solution containing equimolar Ca2+ and EGTA were without effect. Thus, the observed effects of injected EGTA were due to the lowering of the [Ca2+]i. Replacement of e...Continue Reading

Citations

Jan 1, 1985·CRC Critical Reviews in Biochemistry·J I Korenbrot
Oct 15, 1988·European Journal of Biochemistry·A CarettaR T Sorbi
Nov 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·B RayerH Stieve
Jan 1, 1986·Neuroscience Research. Supplement : the Official Journal of the Japan Neuroscience Society·J I Korenbrot, D L Miller

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