PMID: 6540599Jul 17, 1984Paper

Modulation of membrane fusion by ionotropic and thermotropic phase transitions

Biochemistry
N DüzgüneşD Papahadjopoulos

Abstract

We have studied the relationship of ionotropic and thermotropic phase transitions to divalent cation induced fusion of large unilamellar phospholipid vesicles. Fusion was monitored by the Tb/dipicolinic acid fluorescence assay for the intermixing of internal aqueous contents of vesicles. The phase behavior of the membranes was followed by differential scanning calorimetry. (1) Sr2+ and Ba2+ shifted the phase transition temperature (Tc) of bovine brain phosphatidylserine vesicles from 6 to 27 and 31.5 degrees C, respectively. These cations induced vesicle fusion at temperatures above or below the Tc of that cation/phospholipid complex, indicating that an isothermal phase change from the liquid-crystalline to the gel phase is not a requirement for membrane fusion. (2) The temperature dependence of the initial rate of fusion of phosphatidylserine/dipalmitoylphosphatidylcholine (1:1) vesicles in the presence of Ca2+ exhibited a pronounced maximum at 17 degrees C, at the lower part of the broad phase transition endotherm whose Tc was about 25 degrees C; fusion was inhibited completely at 30 degrees C when the membrane was in the liquid-crystalline state. These observations suggest that molecular clusters rich in phosphatidylserine, ...Continue Reading

References

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Citations

Apr 1, 1990·Journal of Bioenergetics and Biomembranes·D PapahadjopoulosN Düzgünes
Jun 15, 1990·Experientia·K N Burger, A J Verkleij
Jun 1, 1986·Chemistry and Physics of Lipids·P R CullisC P Tilcock
Jun 1, 1986·Chemistry and Physics of Lipids·J Wilschut, D Hoekstra
Mar 1, 1990·Chemistry and Physics of Lipids·M C Pedroso de LimaN Düzgüneş
Mar 1, 1991·Chemistry and Physics of Lipids·P K Kinnunen
Dec 20, 1995·Biochimica Et Biophysica Acta·J B Engberts, D Hoekstra
Apr 25, 2000·Progress in Lipid Research·S Nir, J L Nieva
Jan 1, 1997·Free Radical Biology & Medicine·Y J SuzukiA Sevanian
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J BentzF C Szoka
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G Strauss, H Hauser
Jan 1, 1991·Annals of the New York Academy of Sciences·P MeersD Papaphadjopoulos
Apr 16, 1985·Biochemical and Biophysical Research Communications·K D Barfield, D R Bevan
Sep 24, 1985·Biochemistry·D L Daleke, W H Huestis
Sep 24, 1985·Biochemistry·J Bentz, N Düzgüneş
Dec 15, 1987·Biochemistry·N DüzgüneşD Papahadjopoulos
May 2, 1989·Biochemistry·H EllensJ Bentz
Feb 2, 1993·Biochemistry·D E LeckbandJ Israelachvili

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