PMID: 9450626Feb 5, 1998Paper

Modulation of MHC class II+ Langerhans cell numbers in corticosteroid treated epidermis by GM-CSF in combination with TNF-alpha

Experimental Dermatology
G M O'Sullivan, G M Halliday

Abstract

It is important to understand how dendritic cells (DC) are recruited, maintained and stimulated to migrate from tissues to lymph nodes. This is because DC are potent initiators of primary immune responses and candidates for vaccine development. Identification of factors which could lead to increased numbers of DC in tissues could affect immune responses by modulating their interaction with antigen which penetrates the tissue. To identify cytokines which could increase DC in tissues we tested the ability of GM-CSF, TNF-alpha and IL-6 to partially prevent steroid depletion of Langerhans cells (LC) from the epidermis. Cytokines diluted in serum-containing medium were compared with cytokines diluted in albumin-containing, serum-free medium in order to determine a minimum combination of cytokines required to increase LC and the effect of serum on the LC-increasing activity of cytokines. In the presence of serum, GM-CSF or TNF-alpha could increase LC frequency compared to the control; but in the absence of serum neither of these cytokines were effective unless they were combined with each other. In the presence of serum the combination of GM-CSF with TNF-alpha was ineffective. The data support the hypotheses that GM-CSF and TNF-alpha...Continue Reading

References

Nov 1, 1979·Nature·J G FrelingerJ A Frelinger
Nov 1, 1992·The Journal of Investigative Dermatology·I Kimber, M Cumberbatch
May 1, 1992·The Journal of Experimental Medicine·K InabaG Schuler
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Oct 1, 1995·European Journal of Immunology·M MoserO Leo
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K InabaR M Steinman

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Citations

Mar 28, 2003·Journal of Leukocyte Biology·Andrea M Woltman, Cees van Kooten
Dec 21, 2010·Experimental Dermatology·Kathryn J MartiresElma D Baron

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