Modulation of microbial consortia enriched from different polluted environments during petroleum biodegradation

Biodegradation
Rahma OmraniDalila Saidane

Abstract

Environmental microbial communities are key players in the bioremediation of hydrocarbon pollutants. Here we assessed changes in bacterial abundance and diversity during the degradation of Tunisian Zarzatine oil by four indigenous bacterial consortia enriched from a petroleum station soil, a refinery reservoir soil, a harbor sediment and seawater. The four consortia were found to efficiently degrade up to 92.0% of total petroleum hydrocarbons after 2 months of incubation. Illumina 16S rRNA gene sequencing revealed that the consortia enriched from soil and sediments were dominated by species belonging to Pseudomonas and Acinetobacter genera, while in the seawater-derived consortia Dietzia, Fusobacterium and Mycoplana emerged as dominant genera. We identified a number of species whose relative abundances bloomed from small to high percentages: Dietzia daqingensis in the seawater microcosms, and three OTUs classified as Acinetobacter venetianus in all two soils and sediment derived microcosms. Functional analyses on degrading genes were conducted by comparing PCR results of the degrading genes alkB, ndoB, cat23, xylA and nidA1 with inferences obtained by PICRUSt analysis of 16S amplicon data: the two data sets were partly in agree...Continue Reading

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Citations

May 5, 2021·Environmental Science and Pollution Research International·Siddhartha PalSufia K Kazy

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Datasets Mentioned

BETA
PRJNA434054

Methods Mentioned

BETA
PCR
PCA
electrophoresis
amplicon sequencing
PCRs

Software Mentioned

Mothur
STAMP
PICRUSt
Illumina
PICRUSt ( Phylogenetic Investigation of Communities by Reconst...
R
Metastats
kmer
pandaseq

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