Modulation of microRNA processing and expression through RNA editing by ADAR deaminases

Nature Structural & Molecular Biology
Weidong YangKazuko Nishikura

Abstract

Adenosine deaminases acting on RNA (ADARs) are involved in editing of adenosine residues to inosine in double-stranded RNA (dsRNA). Although this editing recodes and alters functions of several mammalian genes, its most common targets are noncoding repeat sequences, indicating the involvement of this editing system in currently unknown functions other than recoding of protein sequences. Here we show that specific adenosine residues of certain microRNA (miRNA) precursors are edited by ADAR1 and ADAR2. Editing of pri-miR-142, the precursor of miRNA-142, expressed in hematopoietic tissues, resulted in suppression of its processing by Drosha. The edited pri-miR-142 was degraded by Tudor-SN, a component of RISC and also a ribonuclease specific to inosine-containing dsRNAs. Consequently, mature miRNA-142 expression levels increased substantially in ADAR1 null or ADAR2 null mice. Our results demonstrate a new function of RNA editing in the control of miRNA biogenesis.

References

Dec 15, 2010·Archives of Neurology·Irfan A Qureshi, Mark F Mehler
May 28, 2011·Accounts of Chemical Research·Chengqi Yi, Tao Pan
Mar 14, 2012·Cell Research·Lijuan Ji, Xuemei Chen
Aug 8, 2012·Molecular Psychiatry·A EranL M Kunkel
Mar 4, 2009·Nature Cell Biology·Julia WinterSven Diederichs
Aug 6, 2013·Nature Communications·Erik Sebastian VikIngrun Alseth
Jun 1, 2006·Nature Genetics·Eugene BerezikovRonald H A Plasterk
Jun 1, 2006·Nature Genetics·Jan KrützfeldtMarkus Stoffel
Dec 18, 2008·Nature Immunology·Hisashi Iizasa, Kazuko Nishikura
May 25, 2010·Nature Reviews. Cancer·Bríd M RyanCurtis C Harris
Dec 22, 2010·Nature Reviews. Genetics·Bjorn-Erik WulffKazuko Nishikura
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Kazuko Nishikura
Jun 21, 2011·Nature Structural & Molecular Biology·Frédérique RauNicolas Charlet-Berguerand
Oct 10, 2006·Oncogene·S Pfeffer, O Voinnet
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Richard XuFengTao Cheng
Jan 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Qingfei JiangCatriona H M Jamieson
Oct 14, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Michael David
Dec 25, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cyril X GeorgeC E Samuel
Oct 10, 2012·Bioinformatics·Alessandro LaganàCarlo M Croce
Jul 1, 2011·Carcinogenesis·Dan DominissiniGideon Rechavi
Jan 24, 2012·Cardiovascular Research·Paula A Da Costa Martins, Leon J De Windt
Sep 25, 2009·Human Molecular Genetics·Glen M BorchertBeverly L Davidson
Aug 3, 2011·Human Molecular Genetics·Cailin E JoyceAnne M Bowcock
Sep 30, 2010·Journal of Biochemistry·Hiroshi I Suzuki, Kohei Miyazono
Dec 20, 2008·Nucleic Acids Research·Zhumur GhoshJayprokas Chakrabarti
Jan 21, 2009·Nucleic Acids Research·Roberto MarcucciFrancisco E Baralle
Jun 3, 2008·Nucleic Acids Research·Rodolphe SuspèneSimon Wain-Hobson
Jul 8, 2008·Nucleic Acids Research·Kirsi PaukkuJukka Y A Lehtonen
Aug 8, 2008·Nucleic Acids Research·Yukio KawaharaKazuko Nishikura
Mar 4, 2009·Nucleic Acids Research·H Alexander EbhardtRichard P Fahlman
Oct 18, 2012·Nucleic Acids Research·Anmol M KiranPavel V Baranov
Jun 1, 2012·The Cancer Journal·Marilena V Iorio, Carlo M Croce
Aug 3, 2006·Genes & Development·J Michael ThomsonScott M Hammond
Mar 9, 2007·Genes & Development·Joel R NeilsonPhillip A Sharp

Citations

Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R W WagnerKazuko Nishikura
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·U KimKazuko Nishikura
Feb 1, 1996·Nature·T MelcherM Higuchi
Dec 13, 1996·The Journal of Biological Chemistry·T MelcherPeter H Seeburg
Feb 11, 2000·RNA·B Masquida, E Westhof
Nov 21, 2001·Nature Reviews. Genetics·L P KeeganM A O'Connell
Dec 18, 2001·EMBO Reports·A D J Scadden, C W Smith
Jun 5, 2002·Annual Review of Biochemistry·Brenda L Bass
Jan 30, 2003·RNA·Mariana Lagos-QuintanaThomas Tuschl
Mar 6, 2003·The Journal of Biological Chemistry·Dan-Sung C ChoKazuko Nishikura
Mar 8, 2003·Science·Lee P LimDavid P Bartel
Aug 9, 2003·Science·Barry HoopengardnerRobert Reenan
Sep 26, 2003·Nature·Amy A CaudyRonald H A Plasterk
Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Nov 14, 2003·The Journal of Biological Chemistry·Qingde WangKazuko Nishikura
Nov 15, 2003·The Journal of Biological Chemistry·Jochen C HartnerPeter H Seeburg
Nov 25, 2003·Science·Elsebet LundUlrike Kutay
Dec 6, 2003·Science·Leath A Tonkin, Brenda L Bass
Dec 6, 2003·Science·Chang-Zheng ChenDavid P Bartel
Dec 19, 2003·Nucleic Acids Research·Sam Griffiths-Jones
Jul 20, 2004·Nature Biotechnology·Erez Y LevanonMichael F Jantsch
Jul 24, 2004·RNA·Daniel J LucianoStefan Maas
Aug 3, 2004·Nature Biotechnology·Kazuko Nishikura
Sep 3, 2004·Genome Research·Dennis D Y KimAbram Gabriel
Sep 17, 2004·Nature·Victor Ambros
Nov 9, 2004·Nature·Richard I GregoryRamin Shiekhattar
Nov 9, 2004·Nature·Ahmet M DenliGregory J Hannon
Nov 10, 2004·PLoS Biology·Alekos AthanasiadisStefan Maas
Nov 24, 2004·The Journal of Biological Chemistry·Weidong YangKazuko Nishikura
Dec 3, 2004·Genes & Development·Jinju HanV Narry Kim
Jan 7, 2005·The EMBO Journal·Alexander G Shearer, Randolph Y Hampton
Mar 23, 2005·Nature Methods·Sébastien PfefferThomas Tuschl
May 17, 2005·Nature Structural & Molecular Biology·A D J Scadden
Jun 24, 2005·Nature·Thimmaiah P ChendrimadaRamin Shiekhattar
Aug 23, 2005·FEBS Letters·Erno Wienholds, Ronald H A Plasterk
Oct 29, 2005·Nucleic Acids Research·A D J Scadden, Mary A O'Connell

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