Modulation of miR-210 alters phasing of circadian locomotor activity and impairs projections of PDF clock neurons in Drosophila melanogaster

PLoS Genetics
Paola CusumanoRodolfo Costa

Abstract

Single microRNAs are usually associated with hundreds of putative target genes that can influence multiple phenotypic traits in Drosophila, ranging from development to behaviour. We investigated the function of Drosophila miR-210 in circadian behaviour by misexpressing it within circadian clock cells. Manipulation of miR-210 expression levels in the PDF (pigment dispersing factor) positive neurons affected the phase of locomotor activity, under both light-dark conditions and constant darkness. PER cyclical expression was not affected in clock neurons, however, when miR-210 was up-regulated, a dramatic alteration in the morphology of PDF ventral lateral neuron (LNv) arborisations was observed. The effect of miR-210 in shaping neuronal projections was confirmed in vitro, using a Drosophila neuronal cell line. A transcriptomic analysis revealed that miR-210 overexpression affects the expression of several genes belonging to pathways related to circadian processes, neuronal development, GTPases signal transduction and photoreception. Collectively, these data reveal the role of miR-210 in modulating circadian outputs in flies and guiding/remodelling PDF positive LNv arborisations and indicate that miR-210 may have pleiotropic effect...Continue Reading

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Citations

Oct 6, 2020·Frontiers in Physiology·Gabriella M MazzottaPaola Cusumano
Jan 24, 2019·Life Science Alliance·Carina M WeigeltLinda Partridge
Oct 21, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiju XiaZhangwu Zhao
Nov 10, 2020·Molecular Neurobiology·Michelle WattsCharles Claudianos
May 11, 2020·Current Biology : CB·Maria P FernandezOrie T Shafer
Jun 1, 2021·Chronobiology International·Geo Anna, Nisha N Kannan

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Datasets Mentioned

BETA
GSE77245

Methods Mentioned

BETA
GTPase
transfection
confocal microscopy
electrophoresis
Feature Extraction
PCR

Software Mentioned

DAVID
7500 Real Time PCR System
TargetScan
SAM
Excel script
Agilent Feature Extraction
ImageJ
DAVID Gene Functional
Drosophila
mirSVR

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