Modulation of mitogen-induced proliferation of autologous peripheral blood lymphocytes by human alveolar macrophages.

Infection and Immunity
H YeagerE Kagan

Abstract

Experiments were carried out to determine the effect of cocultivation of T-cell-enriched human peripheral blood lymphocytes with autologous alveolar macrophages on mitogen-induced proliferation as determined by [(3)H]thymidine uptake. Cells obtained by fiberoptic bronchoscopy and saline bronchial lavage from 14 normal volunteers were enriched for macrophages by adherence in plastic dishes for 1 h in RPMI 1640 medium supplemented with 10% fetal calf serum. Nonadherent mononuclear cells were prepared from heparinized venous blood after Ficoll-Hypaque sedimentation by passage over nylon wool columns. T-cell-enriched populations were incubated with and without alveolar macrophages, either in the presence or absence of phytohemagglutinin. In these experiments, the number of lymphocytes was held constant (10(5) per well), while the number of alveolar macrophages was varied (0.1 x 10(5) to 4.0 x 10(5) per well). Alveolar macrophages generally tended to stimulate phytohemagglutinin-induced lymphoproliferation at lymphocyte/macrophage ratios of 10:1 but consistently and significantly suppressed proliferation at ratios which approach those usually observed in recovered human bronchial lavage fluid, namely, 1:4. The suppressive effect of ...Continue Reading

References

Apr 1, 1979·Cellular Immunology·J J RinehartM E Kaplan
Jan 1, 1978·Immunological Reviews·A C Allison
Oct 1, 1978·The American Review of Respiratory Disease·P G Holt
Jan 1, 1979·Advances in Experimental Medicine and Biology·H B HerscowitzK J Pennline
Oct 11, 1975·The Journal of Clinical Investigation·R P DanieleD T Rowlands
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Aug 1, 1974·The Journal of Clinical Investigation·H YeagerS L Schwartz
Jan 1, 1980·Methods in Cell Biology·G W HunninghakeR G Crystal

❮ Previous
Next ❯

Citations

Jan 1, 1986·Annals of the New York Academy of Sciences·G B ToewsM F Lipscomb
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maike BuettnerSteffen Stenger
Mar 1, 1991·American Journal of Respiratory Cell and Molecular Biology·E A RichJ J Ellner
Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·M YamamotoH Nagura
Dec 1, 1983·Infection and Immunity·M F LipscombJ Stein-Streilein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.