Modulation of MUC7 mucin expression by exogenous factors in airway cells in vitro and in vivo

American Journal of Respiratory Cell and Molecular Biology
Shimin LiL A Bobek

Abstract

The human MUC7 gene encodes a low-molecular-mass mucin that participates in the maintenance of healthy epithelium in the oral cavity, and possibly in respiratory tracts, by promoting the clearance of various bacteria. We examined whether MUC7 gene is expressed in primary normal human tracheobronchial epithelial cells and whether the expression is modulated by exogenous factors. By assessing MUC7 transcripts, we found that the MUC7 gene was induced by culturing the normal human tracheobronchial epithelial cells at the air-liquid interface, in which the cells were well differentiated. When the cells were treated with a panel of cytokines (IL-1beta, IL-4, IL-13, and TNF-alpha), epidermal growth factor, or a bacterial product (Pseudomonas aeruginosa lipopolysaccharide [LPS]), MUC7 transcripts and glycoprotein products were increased 1.7- to 3.2-fold. The effect of LPS on MUC7 gene expression was also studied in the airway tissues of MUC7 gene transgenic mice. In the in vitro cultured trachea and lung explants, the LPS-treated tissues showed over 2-fold increased levels of MUC7 mRNA compared with the untreated specimens. These results were confirmed by in vivo studies using the lungs and tracheas harvested from the transgenic mice i...Continue Reading

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Citations

Oct 27, 2007·Annual Review of Physiology·Philip ThaiReen Wu
Jun 17, 2006·American Journal of Respiratory Cell and Molecular Biology·Shimin Li, Libuse A Bobek
Mar 1, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Neeley RemmersMichael A Hollingsworth
Nov 12, 2009·BioFactors·Satyanarayana RachaganiSurinder K Batra
Jan 31, 2015·Cancer Metastasis Reviews·Suhasini JoshiSurinder K Batra
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