Modulation of Mycobacterium tuberculosis proliferation by MtrA, an essential two-component response regulator.

Molecular Microbiology
Marek FolMalini Rajagopalan

Abstract

Paired two-component regulatory systems consisting of a sensor kinase and a response regulator are the major means by which bacteria sense and respond to different stimuli. The role of essential response regulator, MtrA, in Mycobacterium tuberculosis proliferation is unknown. We showed that elevating the intracellular levels of MtrA prevented M. tuberculosis from multiplying in macrophages, mice lungs and spleens, but did not affect its growth in broth. Intracellular trafficking analysis revealed that a vast majority of MtrA overproducing merodiploids were associated with lysosomal associated membrane protein (LAMP-1) positive vacuoles, indicating that intracellular growth attenuation is, in part, due to an impaired ability to block phagosome-lysosome fusion. A merodiploid strain producing elevated levels of phosphorylation-defective MtrA (MtrA(D53N)) was partially replicative in macrophages, but was attenuated in mice. Quantitative real-time PCR analyses revealed that expression of dnaA, an essential replication gene, was sharply upregulated during intramacrophage growth in the MtrA overproducer in a phosphorylation-dependent manner. Chromatin immunoprecipitation using anti-MtrA antibodies provided direct evidence that MtrA re...Continue Reading

References

Sep 1, 1977·Infection and Immunity·L G Wayne
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E H Lee, A Kornberg
May 1, 1990·Antimicrobial Agents and Chemotherapy·N RastogiH L David
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R B BourretM I Simon
Nov 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L G Wayne
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A HaldimannB L Wanner
Jul 1, 1997·Microbiology·Tanya ParishElaine O Colston
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·K C QuonG T Marczynski
Dec 5, 1998·Journal of Molecular Biology·Y BlatM Eisenbach
Jul 8, 1999·Current Biology : CB·C Stephens
Apr 4, 2000·Current Opinion in Microbiology·J A Hoch
May 10, 2000·Current Biology : CB·W Margolin
Jun 13, 2000·Journal of Bacteriology·T C Zahrt, V Deretic
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·D R ShermanG K Schoolnik
Jul 17, 2001·Molecular Microbiology·E PérezC Martín
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·C M SassettiE J Rubin
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·T C Zahrt, V Deretic
Jul 27, 2002·Annual Review of Microbiology·Gregory T Marczynski, Lucy Shapiro
Dec 14, 2002·Microbiology·Rebecca GreendykeMurty V V S Madiraju

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Citations

May 23, 2012·The Journal of Biological Chemistry·Renata PlocinskaMalini Rajagopalan
Feb 13, 2007·Journal of Bacteriology·Nina MökerSusanne Morbach
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Erik C Hett, Eric J Rubin
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Daniel J BretlThomas C Zahrt
May 11, 2012·Canadian Journal of Microbiology·Mrittika SenguptaRadheshyam K Jayaswal
Jan 5, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Ryutaro Utsumi, Masayuki Igarashi
Feb 23, 2011·Future Medicinal Chemistry·Kunal KumarIwao Ojima
Nov 19, 2011·Tuberculosis·Indumathi S VadrevuMalini Rajagopalan
Dec 17, 2009·Tuberculosis·Manjot KiranMalini Rajagopalan
Dec 17, 2009·Tuberculosis·Manjot KiranMalini Rajagopalan
Apr 17, 2007·Trends in Biochemical Sciences·Rong GaoAnn M Stock
Jul 25, 2015·Molecular Microbiology·Gorla PurushothamMurty V Madiraju
Sep 1, 2006·Molecular Microbiology·Ashwini ChauhanMalini Rajagopalan
Jul 25, 2007·Molecular Microbiology·Anna Zawilak-PawlikAgnés Labigne

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