PMID: 1662905Dec 1, 1991Paper

Modulation of Na-H antiporter activity in human lymphoblasts by altered membrane cholesterol

The American Journal of Physiology
C E Poli de FigueiredoB M Hendry

Abstract

The effects of changes in membrane cholesterol on Na-H antiporter activity in culture human lymphoblasts are described. Lymphoblast cholesterol alteration was achieved with liposomes of phosphatidylcholine (cholesterol depletion) or phosphatidylcholine plus cholesterol (cholesterol enrichment). Lymphoblast intracellular pH (pHi) was examined by fluorimetry using cells loaded with the pH-sensitive dye 2',7'-bis(2-carboxyethyl)5(6)-carboxyfluorescein, and the Na-dependent proton efflux rate at a pHi of 6.0 was taken as the maximum velocity of the Na-H antiporter. Lymphoblast membrane cholesterol depletion activated the Na-H antiporter, and enrichment of membrane cholesterol caused inhibition of the antiporter activity. This study demonstrates that in situ modification of membrane cholesterol can modulate the activity of the Na-H antiporter.

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Citations

Mar 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Martin DworschakJames D Hannon
Aug 15, 2018·Frontiers in Cell and Developmental Biology·Lis C Puga MolinaMariano G Buffone
May 1, 1993·Hypertension·D RosskopfW Siffert
Jul 23, 1998·Biology of Reproduction·N L Cross
Sep 1, 1994·Blood Pressure·E J McMurchieR J Head

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