Modulation of neuronal nitric oxide synthase and apoptosis by the isoflavone genistein in Mdx mice

BioFactors
Sonia MessinaFrancesco Squadrito

Abstract

Dystrophin lack in DMD causes neuronal nitric oxide synthase (nNOS) membrane delocalization which in turn promotes functional muscle ischemia, and exacerbates muscle injury. Apoptosis and the exhaustion of muscle regenerative capacity are implicated in Duchenne muscular dystrophy (DMD) pathogenesis and therefore are relevant therapeutic targets. Genistein has been reported to have pro-proliferative effects, promoting G1/S cell phase transition through the induction of cyclin D1, and anti-apoptotic properties. We previously showed that genistein could reduce muscle necrosis and enhance regeneration with an augmented number of myogenin-positive satellite cells and myonuclei, ameliorating muscle function in mdx mice. In this study we evaluated the underlying mechanisms of genistein effect on muscle specimens of mdx and wild type mice treated for five weeks with genistein (2 mg/kg/i.p. daily) or vehicle. Western blot analysis show that genistein increased cyclin D1 and nNOS expression; and showed an antiapoptotic effect, modulating the expression of BAX and Bcl-2. Our results suggest that this isoflavone might enhance the regenerative spurt in mdx mice muscle restoring nNOS, promoting G1/S phase transition in muscle cell, and inhib...Continue Reading

References

Apr 2, 1998·European Journal of Biochemistry·B Mignotte, J L Vayssiere
Jan 29, 2000·FEBS Letters·Y Tsujimoto, S Shimizu
Feb 24, 2001·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·S EndesfelderA Speer
Feb 8, 2003·Science·Enzo NisoliMichele O Carruba
Jun 6, 2003·Current Opinion in Genetics & Development·Isin Dalkilic, Louis M Kunkel
Apr 2, 2004·Journal of Pediatric Endocrinology & Metabolism : JPEM·Paolo Gianni GiampietroElena Galli
Jul 3, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mina RytenGeoffrey Burnstock
Aug 28, 2004·Endocrinology·Maofu FuRichard G Pestell
Nov 13, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Stefanie EndesfelderAstrid Speer
Jan 6, 2006·The Biochemical Journal·Olivier BassetUrs T Ruegg
Apr 6, 2006·The AAPS Journal·Jayalakshmi SridharNagarajan Pattabiraman
Dec 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Silvia BrunelliEmilio Clementi
Jun 19, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sonia MessinaGiuseppe Vita
Jan 20, 2009·Journal of Agricultural and Food Chemistry·Charlotte RehfeldtMarcus Mau
Mar 7, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Claudia ColussiCarlo Gaetano
May 7, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Clara ScioratiRoberto Latini

❮ Previous
Next ❯

Citations

Jun 2, 2017·Journal of Human Genetics·Akinori Nakamura
Dec 8, 2017·PloS One·Ramzi J KhairallahElaine S Krul
Dec 14, 2017·European Journal of Nutrition·Shinpei TabataKunihiro Sakuma
Feb 13, 2016·Redox Report : Communications in Free Radical Research·Satoru HasegawaKinji Ohno
Feb 26, 2019·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Giuseppe VitaSonia Messina
Oct 12, 2018·PloS One·Gian Luca VitaM'Hammed Aguennouz
May 25, 2020·Molecular and Cellular Biochemistry·Gian Luca VitaSonia Messina

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Gail D ThomasR G Victor
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Sonia MessinaGiuseppe Vita
The Anatomical Record
J P LefaucheurA Sebille
© 2021 Meta ULC. All rights reserved